1. Plant Business Project 1 of 17 Southern Cross Plant ScienceSouthern Cross Plant Science CRC for Remote Economic ParticipationCRC for Remote Economic Participation Plant Business ProjectPlant Business Project Southern Cross Plant ScienceSouthern Cross Plant Science CRC for Remote Economic ParticipationCRC for Remote Economic Participation Plant Business ProjectPlant Business Project

2. Plant Business Project (i) Develop horticultural varieties and methods for creating opportunities for commercialisation of traditionally-used native plants in remote Australia (ii) Use Bush Tomato (Solanum centrale) to develop a model for other plants 2 of 17 Southern Cross Plant ScienceSouthern Cross Plant Science CRC for Remote Economic ParticipationCRC for Remote Economic Participation Plant Business ProjectPlant Business Project Southern Cross Plant ScienceSouthern Cross Plant Science CRC for Remote Economic ParticipationCRC for Remote Economic Participation Plant Business ProjectPlant Business Project

3. Plant Business Project (iii) Develop strategies and recommendations regarding legal instruments and market-oriented approaches for protection of Aboriginal Intellectual Property arising from utilisation of Traditional Knowledge 3 of 17 Southern Cross Plant ScienceSouthern Cross Plant Science CRC for Remote Economic ParticipationCRC for Remote Economic Participation Plant Business ProjectPlant Business Project Southern Cross Plant ScienceSouthern Cross Plant Science CRC for Remote Economic ParticipationCRC for Remote Economic Participation Plant Business ProjectPlant Business Project

4. Plant Business Project Activities Plant Business entails: Aboriginal Community engagement Aboriginal custodian approval Sourcing plant material from traditional lands Gathering plant-related environmental & cultural information Genetic analysis Phytochemical analysis 4 of 17

5. Plant Business Project Activities Plant Business entails: Developing toxicity diagnostic Varietal selection trials & phenotyping Plant breeding pilot study IP protection strategy 5 of 17

6. Plant Business Project Project Map Community Engagement Plant Science TK & IP Protection Field Trials Outcomes improved plant varieties models for industry development robust supply chain & marketing Aboriginal IP protection 6 of 17

7. Plant Business Project Project Map Aboriginal communities CleanGROW Robins Foods Community Engagement Aboriginal communities RemoteBiz Aboriginal custodian approval community engagement sourcing plant material plant info, environment, & stories Community Engagement Plant Science TK & IP Protection Field Trials Outcomes 7 of 17

8. Plant Business Project Project Map Plant Science Southern Cross Univ. genetic analysis developing toxicity diagnostic phytochemical analysis Community Engagement Plant Science TK & IP Protection Field Trials Outcomes 8 of 17

9. Plant Business Project Genetic Analysis 9 of 17 Identifying specific varieties (genotyping) ◦ genomic microsatellite DNA markers Inform variety selection Associate specific plants with traditional lands Support IP protection Distinguishing Solanum species (phylogeny) ◦ chloroplast DNA markers

10. Plant Business Project Phytochemistry 10 of 17 Phytochemical diversity of fruits ◦ investigate plant diversity ◦ distinguish varieties Alkaloid analysis for QA ◦ assist variety selection ◦ develop toxicity test Solanidine Glycosides Sugars MoitiesMol. Wt γ-ChaconineGlucose559.7 β1-Chaconine and β2- Chaconine Glucose, Rhamnose705.9 α-ChaconineGlucose, Rhamnose, Rhamnose852.0 γ-SolanineGalactose559.7 β-SolanineGalactose, Glucose721.9 α-SolanineGalactose, Glucose, Rhamnose868.0 + Sugar(s) Solanidine (Mol. Wt. = 397.6) Solanidine glycoside

11. Plant Business Project Phytochemistry 11 of 17 LCMS Profile of Bush Tomato MeOH Extract UV210 UV280 UV360 MS-APCI (TIC, positive mode)

12. Plant Business Project Phytochemistry 12 of 17 LCMS Profile of Bush Tomato MeOH Extract UV210 UV280 UV360 MS-APCI (TIC, positive mode) MS-APCI EI = 398 Glycoalkaloids

13. Plant Business Project Phytochemistry Total Ion Chromatograms: MeOH Extracts of Bush Tomato at Different Stages of Maturity 13 of 17 Ripe Fruit Dried Fruit Green and Immature Fruit MS-APCI (TIC, positive mode) MS-APCI (TIC, positive mode) MS-APCI (TIC, positive mode) EI = 398

14. Plant Business Project Project Map AZRI Southern Cross Univ. Field Trials plant breeding DUS field trial varietal selection & field trial Community Engagement Plant Science TK & IP Protection Field Trials Outcomes 14 of 17 establish ideotype selection of superior varieties pilot breeding

15. Plant Business Project Project Map Community Engagement Plant Science TK & IP Protection Field Trials Outcomes Southern Cross Univ. Univ. New England TK & IP Protection new plant variety protection ethical strategies legal strategies PhD student project 15 of 17

16. Plant Business Project 16 of 17 protection strategies for Aboriginal and Torres Strait Islander IP expansion of remote enterprises using horticultural varieties of Bush Tomato model for development of other bush plant enterprises employment opportunities and financial independence in remote Australia economic and cultural value of Traditional Knowledge recognised Outcomes

17. Plant Business Project Plant Business Partners Desert Garden Produce Aboriginal Corporation Research providers 17 of 17