1. PREPARATION OF A BACTERIAL SMEAR SPECIAL STAINING TECHNIQUES

2. ASEPTIC PROCECURE FOR MICROBE REMOVAL

3. ASEPTIC PROCECURE FOR
MICROBE REMOVAL FOR
PREPARATION OF A
BACTERIAL SMEAR

6. SPECIMEN PREPARATION FOR LIGHT MICROSCOPY
PREPARATION OF A BACTERIAL SMEAR

7. STAINING
SPECIAL STAINING TECHNIQUES
Negative Staining for Capsules
Capsules
Determinants for virulence in some genera of bacteria
Capsule Staining
Preparation of a bacterial smear along with negative staining w/ Nigrosin or India Ink
Counterstain w/ Crystal Violet

8. STAINING
SPECIAL STAINING TECHNIQUES
Negative Staining for Capsules
Preparing a bacterial smear for Capsule Staining

10. STAINING
SPECIAL STAINING TECHNIQUES
Negative Staining for Capsules
Capsule Stain Results

13. STAINING
SPECIAL STAINING TECHNIQUES
Endospore Stain
Genera of bacteria that produce endospores
Bacillus & Clostridium
Other genera: Desulfotomaculum, Sporosarcina, Sporolactobacillus, Oscillospira, Thermoactinomyces
Schaeffer-Fulton Method for Endospore Staining
Preparation of a Bacterial Smear-Air dry
Application of Primary Dye (Malachite Green) w/ concurrent application of heat
Allow slide to cool
Rinse w/ water
Application of Counterstain (Safranin)

16. SPECIAL STAINING TECHNIQUES Endospore Stain Results
SPECIAL STAINING TECHNIQUES
Endospore Stain Results
Flagellar Stain
Uses mordant and carbolfuchsin stain to increase flagellar diameter until resolved by light microscope