1. NSLS-II Life Science Breakout Session

2. Agenda  Introduction (Miller)  Keynote Speaker: Carolyn Larabell (ALS, UCSF) (30 min)  Technique talks (4 min each)  Imaging  Soft x-ray spectromicroscopy (Jacobsen)  Diffraction Imaging (Jacobsen)  Full-field transmission x-ray microscopy (Larabell)  X-ray fluorescence micro/nanoprobe (Vogt)  Diffraction-enhanced imaging (Zhong)  Microbeam radiation therapy (Dilmanian)  Infrared microspectroscopy and imaging (Miller)  Structural & Molecular Biology:  X-ray footprinting (Chance)  XANES/EXAFS (Chance)  Macromolecular Crystallography (Stojanoff)  Small-Angle X-Ray Scattering (Yang)  Discussion  Closeout (Sweet)

3. 3 BROOKHAVEN SCIENCE ASSOCIATES Life Sciences Discussion Session Summary 1)What are the key scientific drivers? What experiments will NSLS-II enable that are not presently possible? 2)What technical capabilities will these require? (Beamlines, endstations, undulators…) 3)Estimate of community size. 4)What detector requirements does this field have? Do these require R+D? 5)What software and computing infrastructure requirements are there? (Control, data acquisition, analysis) 6)Any particular accelerator requirements? 7)Any particular conventional facility requirements?

4. In identical biological cells: infrared imaging: chemical imaging of biological cells full-field, soft x-ray TXM: nanoscale 3D sub-structure x-ray spectromicroscopy: 3D nanoscale chemical imaging of organic components x-ray fluorescence microprobe: 3D trace element mapping Cross-Technique Imaging Cross-Technique Structural/Molecular Biology For the same protein: small-angle x-ray scattering: low-resolution structure, static & dynamic EXAFS: metal active site environment, static & dynamic MX: atomic-resolution structure, static & dynamic Key Scientific Drivers Life Sciences “Village” Environment

5. Technical Capabilities  Imaging  Soft x-ray spectromicroscopy (ID for tomography)  Diffraction Imaging (ID)  Full-field transmission x-ray microscopy (BM)  X-ray fluorescence micro/nanoprobe (ID)  Diffraction-enhanced imaging (SCW)  Microbeam radiation therapy (ID)  Infrared microspectroscopy and imaging (2 BM)  Structural & Molecular Biology:  X-ray footprinting (3PW)  XANES/EXAFS (DW)  Macromolecular Crystallography (6 IDs)  Small-Angle X-Ray Scattering (1 ID, 1 3PW)

6. Community Size 0 500 1000 1500 2000 2500 3000 19901991199219931994199519961997199819992000200120022003200420052006 Fiscal Year # Users Chemical SciencesMaterials SciencesLife Sciences Environmental and GeoscienceApplied Science and EngineeringOptical/Nuclear/General Physics Unspecified 45% in 2006

7. 45% of NSLS publications are by life sciences users* 65% macromolecular crystallography 30% other structural/molecular biology (spectroscopy, SAXS, footprinting) 5% imaging 0 100 200 300 400 500 600 700 800 900 1000 20022003200420052006 TOTAL Life Sciences Community Size *FY06

8. Quality of Life Offices for beamline staff NEAR beamline; lab space near beamline Sufficient floor space at beamline, AND office like controls environment Laboratories and ancillary facilities Wet lab, with basic equipment (e.g., BSL2, cell culture, crystal growth, protein purif.) Light, epifluorescence, confocal microscopes Sample prep, in particular freezing, microtoming (ultra, cryo, RT) Access to correlated (same mounts ?) electron, IR, and soft x-ray microscopies Adequate staffing !!!!!! Including people with significant background in biological sample prep. Only supporting beamline ops is insufficient, need to support whole experiment (planning, sample prep, data analysis, interpretation)! Life Sciences “Village” Environment Accelerator Meet specs Detectors Limiting today for: MX, EXAFS, IR IT Handling of very large datasets ‘full’ remote control of beamline, remote access; cybersecurity, firewall issues