1. Female and male (wild-type) Sex comb on male No sex comb on female Dark pigmented spot on rounded abdomen of male; male is also usually smaller than female

2. Dead Dead wild-type male: note the wings that are straight off from the body, a sign of death (there are dead flies in other photos as well, by the way)

3. White Sepia Wild Vermilion

4. Bar white-apricot Lobe

5. Eyeless

6. Dichaete

7. Dumpy wings (dp) Sepia Eyes (se)

8. Apterous

9. Vestigial wings, white eyes female

10. Curly wings (cy)

11. Forked Bristle

12. ebony

13. Vestigial Ebony Wild type

14. apterous sepia (ap se) Wild-type fly with wings and red eyes Mutant fly with no wings and dark brown eyes

15. apterous sepia (ap se) Wild-type female: larger than the male, pointed abdomen, no black spot Wild-type male: smaller than the female, rounded abdomen, black spot Two male mutants with no wings and dark eyes

16. dumpy sepia (dp se) Two females, mutant on the left, wild-type on the right Short dumpy wings, different shape from wild-type Note that the mutant also has sepia eyes but in this photo, it is hard to see the color difference from wild-type

17. ebony vestigial (e vg) Wild-type female Mutant female: Note the vestigial (shriveled) wings And dark body color compared to wild-type This is likely a mutant male based on small size and what looks like the black spot on the abdomen, but sex combs would have to be found to be certain

18. sepia (se) Note the different shades of eye color in sepia mutants; the dark color comes in with age so more newly hatched adults might have a lighter brown color that can be mistaken for wild-type red if you’re not careful

19. vestigial sepia (vg se) Two mutant females Wild-type female Wild-type male: Note sex comb on front leg

20. white vestigial (w vg) Mutant male

21. white vestigial (w vg) Mutant male Mutant female Wild-type female Wild-type male

22. yellow Bar (y Bar) Sex comb on wild-type male Wild-type female Sex comb on mutant male Note lighter body color of mutants compared to wild-type Bar-shaped eye

23. yellow (y) Wild-type male Wild-type female Mutant (lighter colored) female Mutant male

24. yellow white miniature (y w m) Wild-type female Sex comb on wild-type male Mutants, likely males but need to Find sex combs to be certain: Note white eyes, lighter color, and tiny size

25. Wild Curly Scalloped

26. Apterous Vestigial Dumpy

27. DichaeteCurved