1. Computational tools for whole-cell simulation Cara Haney (Plant Science) E-CELL: software environment for whole-cell simulation Tomita et al. 1999. Bioinformatics 15(1): 72-84 Mathematical simulation and analysis of cellular metabolism and regulation Goryanin et al. 1999. Bioinformatics 15(9): 749-758

2. Questions addressed in E-CELL Can gene expression, signaling and metabolism be simulated in a manner that will allow one to make predications about a cell? In simplifying a cell, what functions can be sacrificed? What is the minimal gene set?

3. Overview Simple cell based on Mycoplasma genitalium User can define interactions between proteins, DNA and RNA within the cell, etc. as sets of (first order) reaction rules User can observe changes in proteins, etc. M. Genitalium www.nature.come/nsu/010222/010222- 17.html

4. Lists loaded at runtime: –Substances –Rule list –System List Calculates change in concentration of substrates over a user-specified time interval User can select either first-order Euler [error is O(Δt 2 )] or fourth-order Runge-Kutta [O(Δt 5 )] integration methods for each compartment Running the Program

5. Cell Model Hypothetical minimal cell from M. genitalium Only genes essential for metabolism Cell can take up glucose from environment and generates ATP by turning glucose into lactate via glycolysis and fermentation. Lactate is exported from the cell Transcription and translation modeled by including transcription factors, rRNA, tRNA Cell takes up glycerol and fatty acids in order to maintain membrane structure Cell does not replicate

6. Metabolism in the model cell Includes glycolysis, phospholipid biosynthesis, and transcription and translation metabolisms Does not include machinery for replication (DNA replication, cell cycle), amino acid/nucleotide synthesis

7. Classes of Objects Substance –all molecular species within the cell Genes –Modeled as class GenomicElements with coding sequences, protein binding sites and intergenic spacers –Gene class includes transcribed GenomicElements –120 (out of 507) M. genetalium. 7 from other organisms. –includes enzymes to recycle nucleotides and amino acids

8. Genes in the cell Gene typeM. GenOtherTotal Glycolysis Lactate fermentation Phospholipid biosynthesis Phosophotransferase system Glycerol uptake RNA polymerase Amino Acid metabolism Ribosomal L. subunit Ribosomal S. subunit rRNA tRNA tRNA ligase Initiation factor Elongation factor 9 1 4 2 1 6 2 30 19 2 20 19 4 1 0040020000010000400200000100 9 1 8 2 1 8 2 30 19 2 20 4 1 Proteins coding genes RNA coding genes Total 98 22 120 707707 105 22 127

9. Classes of Objects cont. Reaction Rules –One substance turned into another via an enzyme D-fructose 6 phosphate D-fructose 1-6 bisphosphate –Can also represent formation of complexes and movement of substances within the cell –No repressors/enhancers (genes are never turned on or off) although user can specify gene regulation –Each protein and mRNA contain equal proportions of aa’s and nucleotides C0085 + C00002C00354 + C00008 + C00080 [EC 2.7.1.11] ATP ADP + H + 6-phosphofructasokinase

10. Reaction Kinetics Reactions are modeled from EcoCyc and KEGG Non-enzymatic reactions: v = k Π [S i ] v i Enzymatic Reactions (Mechaelis-Menton): V max [S] [S] + K m Also works for a number of substrates and products or formation/degredation of molecular complexes J-1 i v =

11. Virtual Experiments ‘Starve’ cell by decreasing glucose Level of ATP plummets: cell dies ATP initially increases

12. Changes in mRNA levels upon drop of ATP due to Glucose Deprivation

13. Applications Optimization of culture systems Minimal gene set Discover new gene functions Model more complex organisms Genetic engineering Drugs

14. The good and the bad As is, can it tell us anything about the cell? –No repressors/enhancers (genes are not turned on or off) –Cell cannot replicate –No aa/nucleotide biosynthesis Even modified, can it really tell us anything new?

15. Mathematical simulation and analysis of cellular metabolism and regulation Interface for dealing with systems of differential equations. Enter a matrix of equations, has ODE (ordinary differential equation) solver In order to use this for biological applications: –Assumes genome has been sequenced, have gene networks and differential equations of how one gene influences another over time. –Need array of equations specifying how gene A changes with respect to gene B

16. Evaluates over long period of time until steady state is reached within the ‘cell’ Determine relative levels of proteins within a cell Explicit solver –If it is known how much energy is being consumed from these genes undergoing given reactions Implicit solver –If gene X doubles expression, how are all other genes affected? –Can plot change in GeneY as GeneX changes Features

17. More Features Bifurcation Analysis –Chaos, multiple steady states may exist. –Bifurcation points—points where a slight shift in one substance may cause drastic change in steady state Experimental data –Fit your model to experimental data to try and find the best steady state.

18. “It is now feasible to generate a complete metabolic model where complete genome data are available” hmm… Data available is not there at whole cell level. Even if all data is available, can we solve a 6,000 x 6,000 matrix? Just using isolated pathways is this useful? Problems

19. Comparison between two systems Similarities Both use similar approaches to looking at the dynamics of a cell. Both make it possible to ‘knock out’ genes Can make plots to observe changes Differences E-CELL starts from the ground up; builds cell as things are discovered. Math. Sim. Assumes information is there E-CELL only useful for M. genetalium; Can use Math. Sim for any organism and adjust based on experimental data.