1. Harvesting Energy: Glycolysis and Cellular Respiration8
Harvesting Energy:
Glycolysis and Cellular Respiration 1

2. 8.1 How Do Cells Obtain Energy?
Most cellular energy is stored in the chemical bonds of energy-carrier molecules such as adenosine triphosphate (ATP)
Cells break down glucose in two stages: glycolysis, which liberates a small quantity of ATP, followed by cellular respiration, which produces far more ATP

3. 8.1 How Do Cells Obtain Energy?
Photosynthesis is the ultimate source of cellular energy
Photosynthetic organisms capture the energy of sunlight and store it in the form of glucose
Nearly all organisms use glycolysis and cellular respiration to break down sugar molecules to capture energy as ATP

4. 8.1 How Do Cells Obtain Energy?
Photosynthesis is the ultimate source of cellular energy (continued)
Photosynthesis
6 CO2  6 H2O  light energy  C6H12O6  6 O2
Complete glucose breakdown
C6H12O6  6 O2  6 CO2  6 H2O  ATP energy  heat energy

5. energy from sunlight photosynthesis C6H12O6 6 CO2 6 H2O 6 O2 cellular
Figure 8-1 Photosynthesis provides the energy released during glycolysis and cellular respiration
energy from sunlight
photosynthesis
C6H12O6 6
CO2 6
H2O 6 O2
cellular
respiration
glycolysis
ATP 5

6. 8.1 How Do Cells Obtain Energy?
Glucose is a key energy-storage molecule
All cells metabolize glucose for energy
Plants convert glucose to sucrose or starch for storage
In humans, energy is stored as long chains of glucose, called glycogen, or as fat
These storage molecules are converted to glucose to produce ATP for energy harvesting

7. 8.1 How Do Cells Obtain Energy?
Glucose is a key energy-storage molecule (continued)
The breakdown of glucose occurs in phases
Glycolysis
Fermentation
Cellular respiration
During glycolysis and cellular respiration, energy is captured in ATP

8. Figure 8-2 A summary of glucose breakdown
(cytosol)
1 glucose
glycolysis
ATP
2 lactate
2 pyruvate
fermentation
2 ethanol
If O2 is available
If no O2 is available 
2 CO2 6 O2
cellular
respiration
ATP 6
CO2 6
H2O
mitochondrion 8

9. 8.2 What Happens During Glycolysis?
Glycolysis has an etymological root from the Greek, “glyco,” meaning “sweet,” and “lysis,” meaning to “split apart”
Glycolysis begins by splitting glucose (a six-carbon sugar) into two molecules of pyruvate (a three-carbon sugar)
Glycolysis has energy investment and energy harvesting stages

10. 8.2 What Happens During Glycolysis?
Summary of glycolysis
During the energy investment stage, phosphate groups and energy from each of the two ATP are added to glucose to produce fructose bisphosphate
Fructose bisphosphate is broken down into two G3P molecules
During the energy harvesting stage, the two G3P molecules are converted into two pyruvate molecules, resulting in four ATP and two NADH molecules
A net of two ATP molecules and two NADH (high-energy electron carriers) are formed

11. Animation: Glycolysis

12. The essentials of glycolysis
Fig. 8-3

13. 8.3 What Happens During Cellular Respiration?
Cellular respiration breaks down the two pyruvate molecules into six carbon dioxide molecules and six water molecules
The chemical energy from the two pyruvate molecules aids in the production of 32 ATP
Cellular respiration occurs in mitochondria (powerhouses of the cell), organelles specialized for the aerobic breakdown of pyruvate
Mitochondrion has two membranes
The inner membrane encloses a central compartment containing the fluid matrix
The outer membrane forms the outer surface of the organelle, and an intermembrane space lies between the two membranes

14. Figure 8-4 A mitochondrion
matrix
inner membrane
intermembrane space
outer membrane 14

15. 8.3 What Happens During Cellular Respiration?
Cellular respiration occurs in three stages
Pyruvate breakdown
Transfer of electrons along the electron transport chain
Generation of ATP by chemiosmosis

16. Figure 8-5 Reactions in the mitochondrial matrix
Formation of
acetyl CoA 3
NADH 3
NAD
FAD
FADH2
CO2
coenzyme A
coenzyme A
Krebs
cycle
acetyl CoA
pyruvate
NAD
NADH
ADP
ATP 16

17. 8.3 What Happens During Cellular Respiration?
During the first stage of cellular respiration, pyruvate is broken down (continued)
The Krebs cycle
Discovered by Hans Krebs
Hans Krebs won the Nobel Prize in 1953 for his discovery of the Krebs cycle
The Krebs cycle is also known as the citric acid cycle because citrate is produced first in the cycle

18. 8.3 What Happens During Cellular Respiration?
During the first stage of cellular respiration, pyruvate is broken down (continued)
The Krebs cycle (continued)
The Krebs cycle begins by combining acetyl CoA with a four-carbon molecule to form six-carbon citrate, and coenzyme A is released
As the Krebs cycle proceeds, enzymes in the matrix break down the acetyl group, releasing two CO2 molecules and regenerating the four-carbon molecule for use in future cycles

19. 8.3 What Happens During Cellular Respiration?
During the first stage of cellular respiration, pyruvate is broken down (continued)
During the mitochondrial reactions, CO2 is generated as a waste product
CO2 diffuses out of cells and into the blood, which carries it to the lungs, where it is exhaled

20. 8.3 What Happens During Cellular Respiration?
Summing up cellular respiration
In the mitochondrial matrix, each pyruvate molecule is converted into acetyl CoA, producing one NADH per pyruvate molecule and releasing one CO2
As each acetyl CoA passes through the Krebs cycle, its energy is captured in one ATP, three NADH, and one FADH2. The carbons of acetyl CoA are released in two CO2 molecules

21. 8.3 What Happens During Cellular Respiration?
Summing up cellular respiration (continued)
During cellular respiration, the two pyruvate molecules enter the mitochondrion and are completely broken down, yielding two ATP and ten high-energy electron carriers: eight NADH and two FADH2. The carbon atoms from the pyruvates are released in six molecules of CO2
High-energy electrons release energy that is harnessed to pump H into the intermembrane space as they pass through the ETC

22. 8.3 What Happens During Cellular Respiration?
Summing up cellular respiration (continued)
The NADH and FADH2 molecules donate their energetic electrons to the ETC embedded in the inner mitochondrial membrane
These electrons are passed to the ETC, where their energy is used during chemiosmosis to generate a gradient of H, yielding a net of 32 ATP
Energy-depleted electrons exiting the ETC are picked up by H+ released from NADH and FADH2, and combine with oxygen to form water

23. BioFlix Animation: Summary of Cellular Respiration

24. Figure 8-7 The energy sources and ATP harvest from glycolysis and cellular respiration
(cytosol)
1 glucose 2
NADH
glycolysis 2
ATP
2 pyruvate
mitochondrion
(matrix)
CoA 2
NADH 2
CO2
2 acetyl CoA 6
NADH
Krebs
cycle 2
ATP 2
FADH2 4
CO2 O2
H2O
electron transport chain 32
ATP
Total: 36 ATP 24

25. 8.3 What Happens During Cellular Respiration?
Cellular respiration can extract energy from a variety of molecules
Glucose often enters the human body as starch or table sugar, but energy can come from the consumption of fats and proteins in the diet
Intermediate molecules of cellular respiration can be formed by other metabolic pathways
Molecules enter at appropriate stages and then are broken down to produce ATP
Amino acids of protein serve as energy sources

26. 8.3 What Happens During Cellular Respiration?
Cellular respiration can extract energy from a variety of molecules (continued)
Fats are excellent sources of energy
Serve as major energy-storage molecule in animals
Fatty acids combine with CoA then are broken down to produce acetyl CoA molecules, which enter the first stage of the Krebs cycle
A limited intake of fats will allow this process to happen
Overindulgence of fats will cause the body to store excess fat

27. 8.4 What Happens During Fermentation?
Glycolysis is used by virtually every organism on Earth
Earlier forms of life appeared under the anaerobic (no oxygen) conditions existing before photosynthesis
Some microbes lack enzymes for cellular respiration and rely solely on fermentation
Various microorganisms still thrive in places where oxygen is limited or absent
Stomach and intestines of animals
Deep in soil
Bogs and marshes

28. 8.4 What Happens During Fermentation?
Fermentation allows NAD to be recycled when oxygen is absent
If oxygen is not available, the second stage of glucose breakdown is fermentation
Fermentation does not produce any ATP
In fermentation, pyruvate remains in the cytoplasm and is converted into lactate or ethanol  CO2

29. 8.4 What Happens During Fermentation?
Fermentation allows NAD to be recycled when oxygen is absent (continued)
Under anaerobic conditions, with no oxygen to allow the ETC to function, the cell must regenerate the NAD for glycolysis using fermentation
Under aerobic (with oxygen) conditions, NADH donates its high-energy electrons and hydrogen produced in glycolysis to ATP-generating reactions in the mitochondria, ultimately being donated to oxygen during the creation of water and making NAD available to recycle during glycolysis

30. 8.4 What Happens During Fermentation?
Fermentation allows NAD to be recycled when oxygen is absent (continued)
Fermentation does not produce more ATP, but is necessary to regenerate NAD, which must be available for glycolysis to continue
If the supply of NAD were to be exhausted, glycolysis would stop, energy production would cease, and the organism would rapidly die
Organisms use one of two types of fermentation to regenerate NAD
Lactic acid fermentation
Alcohol fermentation

31. 8.4 What Happens During Fermentation?
Some cells ferment pyruvate to form lactate
Lactic acid fermentation produces lactic acid from pyruvate
Muscles that are working hard enough to use up all the available oxygen ferment pyruvate to lactate
To regenerate NAD, muscle cells ferment pyruvate to lactate, using electrons from NADH and hydrogen ions
A variety of microorganisms use lactic acid fermentation, including the bacteria that convert milk into yogurt, sour cream, and cheese

32. Figure 8-8 Glycolysis followed by lactic acid fermentation2
NAD 2
NADH 2
NADH 2
NAD
(glycolysis)
(fermentation)
1 glucose
2 pyruvate
2 lactate 2
ADP 2
ATP 32

33. Figure 8-9a Fermentation in action33

34. 8.4 What Happens During Fermentation?
Some cells ferment pyruvate to form alcohol and carbon dioxide
Many microorganisms, such as yeast, engage in alcohol fermentation under anaerobic conditions
Generates alcohol and CO2 from pyruvate
As in lactic acid fermentation, the NAD must be regenerated to allow glycolysis to continue
During alcohol fermentation, H and electrons from NADH are used to convert pyruvate into ethanol and CO2; this releases NAD, which can accept more high-energy electrons during glycolysis

35. Animation: Fermentation

36. Figure 8-10 Glycolysis followed by alcoholic fermentation2
NAD 2
NADH 2
NADH 2
NAD
(glycolysis)
(fermentation)
1 glucose
2 pyruvate
2 ethanol
2 CO2 2
ADP 2
ATP 36