1. Intermediate Filaments Text and image sources are included using the notes function of this file Cytokeratin Mitochondria DNA

2. The IMF Family TypeProteinHuman gene #Mol WeightCell type Type I (acidic) Type II (basic)Keratins>15 40-57 kDa 53-70 kDaEpithelia Type III (vimentin-like)Vimentin Desmin GFAP Peripherin 11111111 57 kDa 54 kDa 50 kDa 57 kDa Mesenchymal cells Muscle Glia, astrocytes PNS neurons Type IV (neurofilaments)NF-L NF-M NF-H [alpha]- internexin 11111111 62 kDa 102 kDa 110 kDa 66 kDa CNS neurons CNS neurons Type V (nuclear lamina)Lamins A / C Lamins B 1, B 2 1 1 ea 70/63 kDa 67/72 kDa Nucleus - Mature cells Nucleus - Develop.cells * Lens (Type VI)Phakinin/CP49 Filensin1 49 kDa 95 kDaLens Others:Nestin, Synemin, Paranemin, Plasticin, Tanabin... (Prokaryotic IMF family member)(Cresecntin)(Prokaryotes) form heterodimers

3. Assembly as simple as abcdefg central rod domain bundles of 8 tetramers

4. Atomic Model of the Dimer

5. Keratins Lamins Cytokeratins in Epithelial Cells

6. Epidermolysis Bullosa Simplex arises from keratin point mutations Dowling-Meara - widespread blistering Koebner - blistering confined to hands and feet Weber-Cockayne - least severe

7. Stretching an EBS cell destroys the keratin cytoskeleton

8. Vimentin in Fibroblasts

9. Cancer detection Cytokeratins are normally expresssed in skin but not in the underlying tissues. Antibody staining can reveal hyperplasia and metastasis of epithelial cells into those tissues as a cancer progresses. Normal Skin Squalous cell carcinoma

10. Neurofilaments in Neurons NF-H in neurons GFAP in glia

11. Active Remodeling (note - short clips are repeated several times in each part of the sequence)

12. Cytokeratin- GFP dynamics 4 hour sequence

13. Cytokeratin-GFP dynamics during mitosis 40 minute sequence

14. Okadaic acid (phosphatase inhibitor) results in cytokeratin disassembly 200+ minute sequence Cytokeratin 13::GFP

15. Cytokeratin-GFP dynamics require ATP 96 minute sequence

16. Cytokeratin- GFP dynamics requires microtubules 115 minute sequence

17. Microinjection of anti-detyrosinated tubulin antibodies collapses the IMF network

18. Cytokeratin- GFP dynamics doesn’t require micro- filaments 115 minute sequence

19. Vimentin-GFP Time-lapse Close-up cell edge Nocodazole collapse

20. Vimentin overlays Distinct from Microfilaments Co-align with Microtubules

21. Plectin links Vimentin IMFs and Microtubules

22. The Cytomatrix

23. Tensegrity F-actin

24. The Axonal Matrix

25. Murine Macrophage

26. Nocodozole- treatment depolymerizes mts and causes lamellipodial retraction