1. The Cytoskeleton

8. Intermediate Filaments 8-10 nm in diameter Four types: basis of aa sequence actin & tubulin -> globular protein, cell type specific subunit of IF -> fibrous protein, wide range of size, nonpolar Around nucleus, extend out to cell periphery (by attachment of ut) Abundant in cells require mechanical stress: epithelia, length of axon, cytoplasm of smooth muscle

14. Epidermolysis bullosa simplex

15. Amyotrophic lateral sclerosis (Lou Gehrig’s Disease) : accumulation and abnormal assembly of NF in motor neuron cell bodies and in the axon -> interfere axonal transport -> degeneration of axon -> muscle weakness and atrophy

21. A microtubule array can find the center of a cell Dynamic + ends pointing outward and stable - ends collected the center

26. Dynamic instability

30. Effects of stathmin on microtubule polymerization

31. Organization of microtubule bundles by MAPs

32. The effects of proteins that bind to microtubule ends

33. Capping proteins help to localize microtubules

34. Microtubule motors Dynein Kinesin

35. Microtubules in cilium or flagellum

36. Ciliary dynein

37. The bending of an axoneme

38. Basal bodies

40. Kinesin and KRP

43. A model for the attachment of dynein to a membrane-enclosed organelle

47. Actin filaments

52. The tip of the leading edge of a cell nucleates actin filaments

53. Nucleation by the ARP complex

54. Web formation by the ARP complex

55. Actin Binding Proteins

57. Profilin bound to an actin monomer

58. Effects of thymosine and profilin on actin polymerization

60. Filament capping and its effects on filament dynamics

61. Actin array in a cell

63. Actin-cross-linking proteins

64. The formation of two types of actin filament bundles

65. A microvillus

66. Filamin cross-links actin filaments into a 3-D network with the physical properties of a gel

67. Filament severing and its effects on filament dynamics

68. A model for actin filament severing by gelsolin

71. Platelet activation

73. The role of ERM (ezrin, radixin, moesin)-family proteins in attaching actin filaments to the plasma membrane

74. Focal contacts and stress fibers in a cultured fibroblast

75. The effects of Rac, Rho, and Cdc42 on actin organization in fibroblasts

76. The Actin motors Myosin

77. Myosin II

78. The Myosin II bipolar thick filament

79. The motor activity of the myosin head

80. Myosin superfamily tree

81. Comparison of the domain structure of the heavy chains of myosin types

84. Light-chain phosphorylation and the regulation of the assembly of myosin II into thick filaments

85. Skeletal muscle myofibrils

86. Organization of accessory proteins in a sarcomere

89. T tubules and the sarcoplasmic reticulum