1. Hemoglobin Experiment
In the hemoglobin experiment, blood was mixed with potassium ferricyanide in a test tube. This tube is then placed in a spectrophotometer (seen above) and the amount of cyanmethemoglobin is determined by measuring the amount of transmittance (%T) of light that passes through the test tube. The %T can be used to determine the number of grams of hemoglobin that is in 100 ml of that blood. (See problem on slide 3.)

3. (answers are on following slide)
PROBLEM
(answers are on following slide)
1. If the %T is 44, how many grams of hemoglobin can 100 ml of this blood carry? (See chart on slide 2.)
How many ml of O2 can this same blood carry?
If the %T is 30, how many grams of hemoglobin can 100 ml of this blood carry? (See chart on slide 2, look at the calculation at the bottom.)

4. PROBLEM
If the %T is 44, how many grams of hemoglobin can 100 ml of this blood carry? (See chart on slide 2.)
14.3 g
How many ml of O2 can this same blood carry?
14.3 g (Hgb) X 1.34 = ml O2
If the %T is 30, how many grams of hemoglobin can 100 ml of this blood carry? (See chart on slide 2, look at the calculation at the bottom
(100%-30%) x = g
x 1.34 = ml O2