1. Activity Evolution: a change in populations over successive generations. Complete an Evidence Summary Chart as a class. Identify one pro (positive) and one con (negative) argument for each area of evidence in the graphic organizer and one example for each of the two areas you were assigned. Define the key terms related to each area of evidence.

2. Evidence for Evolution Area: Embryology Examples: embryo of pig and human Pro: best evidence because it is the most fundamental or basic information Vocabulary:embryo — early stages of developing plants and animals comparative embryology — study of different developing organisms.

3. Comparative Embryology

5. Evidence for Evolution Area: Biochemistry Examples: amino acid sequence of chimpanzee and human Con:limited access to amino acids of past organisms Vocabulary:comparative biochemistry — comparing amino acid sequences of different organisms

6. If two organisms have similar DNA molecules, they have similar proteins. Similar proteins have similar amino acid sequences (orders). Thus, if amino acid sequences are similar, DNA of the organisms is similar. Scientists believe that similar DNA sequences indicate a common origin. The more similar the DNA of two living organisms, the more closely related they may be to one another. Hemoglobin, a protein in red blood cells, has been studied. Scientists know the specific amino acids and their arrangements in hemoglobin molecules of humans, gorillas, and horses. Biochemical Evidence for Evolution

7. Biochemical Evidence for Evolution DNA Sequence -------> Amino Acid Sequence -------> Protein C A C G T T -------> valine histidine -------> Hemo. Hemoglobin Amino Acid Sequence: Human:ValHisPro Gorilla:ValHisGly Horse:ValHisPro This sample size is too small to provide accurate data. One must look at the entire amino acid sequence. The more similar the sequences, the more closely related the organisms.

8. In this investigation, you will: (a)count and record differences in the sequence of amino acids in similar portions of human, gorilla, and horse hemoglobin. (Table 1) (1) (2) (3) (b)count and record the molecules of each amino acid present in similar portions of human, gorilla, and horse hemoglobin. (Table 2) (1) (2) (3) (c)use these data to show how biochemical evidence can be used to support evolution.

9. Table 1. Number of Amino Acid Sequence Differences OrganismsNumber of Differences (1) Gorilla and Human (2) Horse and Human (3) Gorilla and Horse Biochemical Evidence for Evolution

10. Table 1 – differences in sequence

11. Table 2. Number of Each Amino Acid Amino AcidAbbreviation(1) Human(2) Gorilla(3) Horse AlanineAla ArginineArg Aspartic Acid Asp CysteineCys Biochemical Evidence for Evolution * Each column should total 146

12. Table 2 - # of each type of amino acid

13. Biochemical Evidence for Evolution 1.DNA codes for amino acids. 2.Amino acid chains form proteins. 3.Proteins perform specific functions. 4.Hemoglobin is a protein in red blood cells that carries oxygen. 5.Hemoglobin is found in all mammals. 6.It is similar in its amino acid sequence but not identical.

14. Amino Acid Sequence for Hemoglobin Molecule

15. Human (H)Chimp (C) Chromosome comparison

16. Human (H) Chimp (C)Orangutan (O) Gorilla (G) Chromosomes of Primates

18. What is a Mutation? A mutation is a permanent change in the DNA sequence (ATCG) of a gene. Mutations in a gene's DNA sequence can alter the amino acid sequence of the protein encoded by the gene. How does this happen? Like words in a sentence, the DNA sequence of each gene determines the amino acid sequence for the protein it encodes. The DNA (ATCG) sequence is interpreted in groups of three nucleotide bases, called codons. Each codon specifies a single amino acid in a protein.

19. Mutate a sentence! We can think about the DNA sequence of a gene as a sentence made up entirely of three-letter words. In the sequence, each three-letter word is a codon, specifying a single amino acid in a protein. Have a look at this sentence: Thesunwashotbuttheoldmandidnotgethishat. If you were to split this sentence into individual three-letter words, you would probably read it like this: The sun was hot but the old man did not get his hat. This sentence represents a gene.

20. This sentence represents a gene. Each letter corresponds to a nucleotide base, and each word represents a codon. What if you shifted the three-letter "reading frame?" You would end up with T hes unw ash otb utt heo ldm and idn otg eth ish at. Or Th esu nwa sho tbu tth eol dma ndi dno tge thi sha t. As you can see, only one of these three "reading frames“ translates into an understandable sentence. In the same way, only one three-letter reading frame within a gene codes for the correct protein.

21. Mutation in Amino Acid Sequence for Hemoglobin Molecule

22. Evidence for Evolution Area: Genetics Examples: domesticated plants and animals; DNA sequence; mutations Pro:changes easily seen in domestic plants/animals; gene links for generation after generation documented. Con:limited access to past DNA Vocabulary:population — all organisms within a breeding group. selective breeding — artificial selection of only the most desirable traits to be bred.

23. Domesticated Plants and Animals

25. cattlecats

26. Domesticated Plants and Animals corn apples

27. Domesticated Plants and Animals Selective (artificial) Breeding

28. Certificate of Pedigree

29. Domesticated Plants and Animals Selective (artificial) Breeding

30. Certificate of Pedigree

32. Human (H)Chimp (C) Chromosome comparison

33. Human (H) Chimp (C)Orangutan (O) Gorilla (G) Chromosomes of Primates

35. DNA Base Sequence Comparisons