1. Introduction to the Microbiology Lab
Exercises 1-2, 2-1, 3-1, and 3-3

2. Laboratory Safety

3. Bio Safety Levels

4. Student Conduct
Do NOT smoke, eat, drink, or bring food or drinks into the laboratory room
Do NOT apply cosmetics or handle contact lenses
Wash your hands thoroughly with soap and water after handling microbes AND before leaving the laboratory room each day
Do NOT remove organisms or chemicals from the laboratory
Work carefully and methodically
COME PREPARED TO LAB FOR THAT DAY’S WORK

5. Basic Laboratory Safety
Wear your lab coat and gloves while working in the laboratory
Remove them before leaving
Discard gloves appropriately (not in standard trash)
NO OPEN-TOED SHOES OR SANDALS
Turn off Bunsen burners when not in use
Secure loose hair and clothing when working with an open flame
Never pipette by mouth

6. Safety Equipment Know the location of the First Aid Kit
Know the location and proper way to use the Fire Blanket, Shower, and Fire Extinguisher
Know the location and proper way to use the Eye Wash Basin
NOTIFY YOUR INSTRUCTOR OF ANY EVENT THAT NECESSITATES THE USE OF THESE

7. Reduce Risk of Contamination
Clean bench with disinfectant before and after lab
70% alcohol can also be used as a disinfectant
Allow to air dry rather than wipe up
Never lay culture tubes on bench top
To clean spills: cover with paper towels, saturate with disinfectant and leave for 20 minutes
Discard in appropriate container
Place non-essential books, etc. out of the way of your experiments

8. Disposing of Contaminated Materials
Broken glassware, needles, wooden cotton swabs, etc. MUST be placed in the “sharps” container
Culture tubes are to have any labels removed and placed upright in racks on the “kill” cart
Petri dishes can be discarded into solid waste on the “kill” cart WITHOUT removing the labels
Gloves are to be discarded in the appropriate biohazard containers

9. Care of Microscopes Microscopes are located at each lab station
Following their use, microscopes:
Should have all oil removed from the lenses using LENS PAPER and cleaning solution
YOUR INSTRUCTOR WILL INSPECT THEM BEFORE YOU ARE PERMITTED TO LEAVE
Scanning (4X) lens should be in place
Stage should be at lowest setting
Rheostat should be adjusted to its lowest setting
Cord should be wrapped carefully around the base and not “flopping” around

10. Care of the Lab Place seats back under benches before you leave
Wipe down the sink-tops with paper towel if you are among the last students in the lab
Remove any paper towels left behind by less conscientious students
THE LAB SHOULD LOOK AS NICE AFTER YOU ARE DONE AS IT DID BEFORE YOU STARTED

11. Scavenger Hunt

12. Essential Equipment Security Phone Disinfectant
Antiseptic Hand Solution
Fire Extinguisher
Emergency Shower
With Eye Wash
Fire Blanket
MSDS Folder
Biohazard Waste Container
Biohazard Sharps Container
Broken Glass Container
“Kill” Cart
Incubators
Refrigerated Cold Room
Biological Safety Cabinet
Vortex Mixers
Microscopes
First Aid Kit
Chemical Spill Kit
Microbiology Lab Tools
Autoclave
Fire Alarm
Escape Routes
Gas Shut-off

13. New Experiments

14. GLO-Germ Hand Wash Sanitation System
Exercise 1-2

15. Glo Germ Hand Wash System
Effective hand washing minimizes transmission of pathogens by health-care workers and food handlers
How Effective Is Your Hand Washing Technique?
Follow the directions outlined in the lab manual…
Work in groups of 2 with one person applying the Glo Germ gel and the other person holding the UV lamp
Do NOT hold UV lamp if you also have Glo Germ applied
TURN UV LAMP OFF WHEN EXERCISE IS COMPLETE

16. The Ubiquity of Microorganisms
Exercise 2-1

17. Ubiquity of Microorganisms
Many microbes can easily be isolated from a variety of environments
Soil, water, plants, and animals
Just how ubiquitous are microbes?
Follow the directions outlined in the lab manual…
You will work in groups of 4
Incubate plates 1, 2, and 8 at 25oC.
Incubate plates 3, 4, 5, 6, and 7 at 37oC.

18. Introduction to the Light Microscope
Exercise 3-1

19. Principles of Light Microscopy
Illumination
Stains add contrast to specimens
Magnification
Compound microscope has paired ocular lenses and an objective lens
Resolution (Resolving Power)
Clarity of an object viewed under the microscope
Distance that two points must be apart to appear as two distinct points
Resolution of typical light microscope is 0.2μm

20. Parts of the Light Microscope
Involved in Illumination
Involved in Magnification/Focus
Base
On/Off Switch
Lamp (Bulb)
Light Intensity Knob
Sub-stage Condenser
Iris Diaphragm
Stage
Stage Mechanisms
Course/Fine Adjustment
Revolving Nosepiece
Objective Lenses
4X, 10X, 40X
100X (Oil Immersion)
Ocular Lenses

21. Microscopic Examination of Eukaryotic Microbes
Exercise 3-3

22. Examination of Eukaryotic Microbes
Follow the directions outlined in the lab manual…
You will work individually with your own microscope, but feel free to divide the specimens up equally for purposes of seeing everything:
Oscillatoria sp. Amoeba
Saccharomyces cerevisiae Giardia lamblia
Aspergillus sp. Spyrogyra sp.
Rhizopus sp. Diatoms
Euglena sp. Volvox sp.
Paramecium caudatum Dog tapeworm
Human louse Plasmodium sp. (in RBCs)