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Published byAllyson Price Modified over 9 years ago
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Physical insulation of pathways or functions
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How to build complex synthetic circuits? Electronic hard wired: reusability of components. Biology is diffusive: crosstalk We need to find a way to insulate the different components circuits.
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Pathway insulation methods Orthogonality (chemical insulation) Family of orthogonal parts Physical insulation Scaffolding Synthetic organelles Synthetic ecosystems
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PROJECT 2: BUILDING A NEW ORGANELLE FOR SYNTHETIC BIOLOGY STEP 1: Create spatially distinct membrane compartment that has unique molecular identity wild-type cellcreate “synthesome”use “synthesome” STEP 2: Recruit proteins to carryout any synbio process of choice (e.g. drug or biofuel factory) A quick recap
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An external organelle External organelles producing cell Targetting Components of a pathway to secretion External organelles producing cell
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Chassi s (Feeder) External organelle An external organelle
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Chassi s (Feeder) Attachment transmembrane proteins An external organelle
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External organelle Chassi s (Feeder)
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External organelle Chassi s (Feeder) communication
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External organelle composition (1) Chassi s (Feeder) factory Receiver sender Chassis (Feeder) Function?
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Advantage: part reusability Chassi s (Feeder) Chassis (Feeder) State output Example: multicolor Hasty oscillator output
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External organelle composition (2) Chassi s (Feeder) pathway Starting product
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Natural example: Nanoarchaeum equitans
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Nanoarchaeum: ABC transporters Chassi s (Feeder) Communication: Within eukaryotes, ABC-transporters mainly transport molecules to the outside of the plasma membrane or into membrane-bound organelles such as the endoplasmic reticulum, mitochondria, etc.
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Gap junctions Bacterial conjugation Natural examples
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