2. AP Biology 2007-2008 The Cell Membrane

3. AP Biology 2 The Structure of A Cell  ALL cells have a cell membrane  A thin, flexible barrier around the cell  Separates the inside of the cell from the outside

4. AP Biology  Controls traffic in & out of the cell  Acts like a “gate”  selectively permeable  allows some substances to cross more easily than others  hydrophobic vs hydrophilic  Made of phospholipids, proteins & other macromolecules Structure of a Cell

5. AP Biology 4 The Cell Membrane  Structure  2 layers of PhosphoLipids = Phosphate + lipid  2 fats (lipids) as the tail that “hate water” – hydrophobic  1 phosphate molecule as the head that “loves water” – hydrophilic  Faces the water inside the cell  This is referred to as a “Lipid Bilayer”  Proteins

6. AP Biology Phospholipids Fatty acid Phosphate  Fatty acid tails  hydrophobic  Phosphate group head  hydrophilic  Arranged as a bilayer Aaaah, one of those structure–function examples

7. AP Biology Phospholipid bilayer polar hydrophilic heads nonpolar hydrophobic tails polar hydrophilic heads

8. AP Biology Chapter 4 – Membrane Structure & Function Plasma membrane Structure & Function Phopholipids, Glycolipids, Proteins, Glycoproteins, Cholesterol

9. AP Biology Membrane is also proteins, cholesterol. Extracellular fluid Cholesterol Cytoplasm Glycolipid Transmembrane proteins Filaments of cytoskeleton Peripheral protein Glycoprotein Phospholipids

10. AP Biology Membrane Proteins  Membrane proteins:  peripheral proteins  loosely bound to surface of membrane  cell surface identity marker (antigens)  integral proteins  penetrate lipid bilayer, usually across whole membrane  transmembrane protein  transport proteins  channels, pumps

11. AP Biology Many Functions of Membrane Proteins Outside Plasma membrane Inside Transporter Cell surface receptor Enzyme activity Cell surface identity marker Attachment to the cytoskeleton Cell adhesion

12. AP Biology Membrane carbohydrates  Play a key role in cell-cell recognition  ability of a cell to distinguish one cell from another  antigens  important in organ & tissue development  basis for rejection of foreign cells by immune system

13. AP Biology 2007-2008 Movement across the Cell Membrane

14. AP Biology Diffusion  Diffusion  movement from high  low concentration  Diffusion  movement from high  low concentration

15. AP Biology Diffusion  Move from HIGH to LOW concentration  “passive transport”  no energy needed diffusionosmosis movement of water

16. AP Biology Diffusion across cell membrane  Cell membrane is the boundary between inside & outside…  separates cell from its environment IN food carbohydrates sugars, proteins amino aids lipids salts, O 2, H 2 O OUT waste ammonia salts CO 2 H2OH2O products cell needs materials in & products or waste out IN OUT Can it be an impenetrable boundary? NO!

17. AP Biology Diffusion through phospholipid bilayer  What molecules can get through directly?  fats & other lipids inside cell outside cell lipid salt aa H2OH2O sugar NH 3  What molecules can NOT get through directly?  polar molecules H2OH2O  ions  salts, ammonia  large molecules  starches, proteins

18. AP Biology Channels through cell membrane  Membrane becomes semi-permeable with protein channels  specific channels allow specific material across cell membrane inside cell outside cell sugaraa H2OH2O salt NH 3

19. AP Biology 2007-2008 The Special Case of Water Movement of water across the cell membrane

20. AP Biology Osmosis is diffusion of water  Water is very important to life, so we talk about water separately  Diffusion of water from high concentration of water to low concentration of water  across a semi-permeable membrane

21. AP Biology Concentration of water  Direction of osmosis is determined by comparing total solute concentrations  Hypertonic - more solute, less water  Hypotonic - less solute, more water  Isotonic - equal solute, equal water hypotonichypertonic water net movement of water

22. AP Biology freshwaterbalancedsaltwater Managing water balance  Cell survival depends on balancing water uptake & loss

23. AP Biology Managing water balance  Isotonic  animal cell immersed in mild salt solution  example: blood cells in blood plasma  problem: none  no net movement of water flows across membrane equally, in both directions  volume of cell is stable balanced

24. AP Biology Managing water balance  Hypotonic  a cell in fresh water  example: Paramecium  problem: gains water, swells & can burst  water continually enters Paramecium cell  solution: contractile vacuole  pumps water out of cell  ATP  plant cells  turgid freshwater ATP

25. AP Biology Managing water balance  Hypertonic  a cell in salt water  example: shellfish  problem: lose water & die  solution: take up water or pump out salt  plant cells  plasmolysis = wilt saltwater

26. AP Biology  STOP HERE!!!!

27. AP Biology Facilitated Diffusion  Diffusion through protein channels  channels move specific molecules across cell membrane  no energy needed “The Bouncer” open channel = fast transport facilitated = with help high low

28. AP Biology Active Transport “The Doorman” conformational change  Cells may need to move molecules against concentration gradient  shape change transports solute from one side of membrane to other  protein “pump”  “costs” energy = ATP ATP low high

29. AP Biology Aquaporins  Water moves rapidly into & out of cells  evidence that there were water channels 1991 | 2003 Peter Agre John Hopkins Roderick MacKinnon Rockefeller

30. AP Biology Cell (compared to beaker)  hypertonic or hypotonic Beaker (compared to cell)  hypertonic or hypotonic Which way does the water flow?  in or out of cell.05 M.03 M Osmosis…

31. AP Biology symportantiport Active transport  Many models & mechanisms ATP

32. AP Biology Getting through cell membrane  Passive Transport  Simple diffusion  diffusion of nonpolar, hydrophobic molecules  lipids  high  low concentration gradient  Facilitated transport  diffusion of polar, hydrophilic molecules  through a protein channel  high  low concentration gradient  Active transport  diffusion against concentration gradient  low  high  uses a protein pump  requires ATP ATP

33. AP Biology Transport summary simple diffusion facilitated diffusion active transport ATP

34. AP Biology How about large molecules?  Moving large molecules into & out of cell  through vesicles & vacuoles  endocytosis  phagocytosis = “cellular eating”  pinocytosis = “cellular drinking”  exocytosis exocytosis

35. AP Biology Endocytosis phagocytosis pinocytosis receptor-mediated endocytosis fuse with lysosome for digestion non-specific process triggered by molecular signal