1. The Cell Membrane

2. Overview Cell membrane separates living cell from nonliving surroundings – thin barrier = 8nm thick Controls traffic in & out of the cell – selectively permeable (or semi-permeable) – allows some substances to cross more easily than others hydrophobic vs hydrophilic Made of phospholipids, proteins & carbohydrates

3. Phospholipids Fatty acid tails – hydrophobic Phosphate group head – hydrophilic Arranged as a bilayer Fatty acid Phosphate

4. Phospholipid bilayer polar hydrophilic heads nonpolar hydrophobic tails polar hydrophilic heads Are they saturated or unsaturated fat molecules?

5. More than lipids… In 1972, S.J. Singer & G. Nicolson proposed that membrane proteins are inserted into the phospholipid bilayer. They called it the Fluid Mosaic Model.

6. Membrane is a collage of proteins & other molecules embedded in the fluid matrix of the lipid bilayer Extracellular fluid Cholesterol Cytoplasm Glycolipid Transmembrane proteins Filaments of cytoskeleton Peripheral protein Glycoprotein Phospholipids

7. Membrane Proteins Proteins determine membrane’s specific functions – cell membrane & organelle membranes each have unique collections of proteins Membrane proteins: – peripheral proteins loosely attached to the surface of the membrane – integral proteins stick into the lipid bilayer usually go all the way across the whole membrane

8. Many Functions of Membrane Proteins Outside Plasma membrane Inside Transporter Cell surface receptor Enzyme activity Cell surface identity marker Attachment to the cytoskeleton Cell adhesion

9. Membrane carbohydrates Play a key role in cell-cell recognition – ability of a cell to distinguish one cell from another – important in organ & tissue development – basis for rejection of foreign cells by immune system

10. Check Your Understanding… 1.What does semi-permeable mean? 2.Which biomolecule makes up membranes? 3.What do proteins do in membranes? 4.What do carbohydrates do on membranes? 5.Why do we use the “fluid mosaic model” to describe membranes?

11. Movement across the Cell Membrane

12. Diffusion 2nd Law of Thermodynamics – universe tends towards disorder (entropy)  Diffusion  movement from high  low concentration  Diffusion  movement from high  low concentration

13. Diffusion Move from HIGH to LOW concentration – “passive transport” – no energy needed diffusion

14. Diffusion across cell membrane Cell membrane is the boundary between inside & outside… – separates cell from its environment IN food carbohydrates sugars, proteins amino acids lipids salts, O 2, H 2 O OUT waste ammonia salts CO 2 H 2 O products cell needs materials in & products or waste out IN OUT Why do molecules need to cross the membrane?

15. Diffusion through the phospholipid bilayer Which molecules can get through directly? – fats & other lipids inside cell outside cell lipid salt aa H2OH2O sugar NH 3  What molecules can NOT get through directly?  polar molecules  H 2 O  ions  salts, ammonia  large molecules  starches, proteins

16. Channels through cell membrane Membrane becomes semi-permeable with protein channels – specific channels allow specific material across cell membrane inside cell outside cell sugaraa H2OH2O salt NH 3

17. Facilitated Diffusion Diffusion through protein channels – channels move specific molecules across cell membrane – no energy needed “The Bouncer” open channel = fast transport facilitated = with help high low

18. Active Transport Cells may need to move molecules against the concentration gradient (from low concentration to high concentration) – shape change transports the molecule from one side of membrane to other – protein “pump” – “costs” energy = ATP ATP

19. Getting through the cell membrane Passive Transport – Simple diffusion diffusion of nonpolar, hydrophobic molecules – lipids – high  low concentration gradient – Facilitated transport diffusion of polar, hydrophilic molecules through a protein channel – high  low concentration gradient Active transport – diffusion against concentration gradient low  high – uses a protein pump – requires ATP ATP

20. Transport summary simple diffusion facilitated diffusion active transport ATP

21. How about large molecules? Moving large molecules into & out of cell – through vesicles & vacuoles endocytosis exocytosis

22. Endocytosis phagocytosis pinocytosis receptor-mediated endocytosis fuse with lysosome for digestion non-specific process triggered by molecular signal

23. Diffusion Review Which molecules can move through membranes without help? What is the difference between passive and active transport? If molecules can move across the membrane, why do our cells still need vesicles?

24. The Special Case of Water Movement of water across the cell membrane 2007-2008

25. Osmosis is… when water moves across a membrane to balance concentrations.

26. Concentration of water Direction of osmosis is determined by comparing total solute concentrations – Hypertonic - more solute, less water – Hypotonic - less solute, more water – Isotonic - equal solute, equal water hypotonichypertonic water net movement of water

27. freshwaterbalancedsaltwater Managing water balance Cell survival depends on balancing water uptake & loss

28. Check for Understanding At the bottom of your notes, explain what would happen if you took a fish from the ocean and put it in a freshwater lake. Use the words: – concentration – osmosis – membrane – semi-permeable