1. best practices in laboratories from the dual perspectives of research and sustainability

2. best practices: laboratory equipment fume hoods ultra-low freezers lasers microscopes large centrifuges heating blocks tissue culture hoods autoclaves deionized, distilled, and ultrapure water

3. plastics gloves animal bedding best practices: laboratory consumables

4. a brief conceptualization of energy consumption 30 kWh/day

5. fume hoods wikipedia.com

6. fume hoods greenfumehood.com

7. fume hoods classification velocity of airflow through the hood uses of the hood ducted v ductless

8. fume hoods: how it works ehs.ufl.edu

9. fume hoods: how it works web.princeton.edu

10. fume hoods: constant air volume (CAV) exhaust flowrate or quantity of air pulled through the hood is constant oregonstate.edu

11. fume hoods: variable air volume (VAV) exhaust flowrate or quantity of air pulled through the hood varies southwestsolutions.com

12. fume hoods energy consumption 3x typical household -Lawrence Berkeley Labs

13. ‘be good in the hood’

15. ALWAYS close the sash thermoscientific.com safest position.

16. ‘be good in the hood’ do not store unnecessary items in the hood

17. ‘be good in the hood’ being good in the fume hood not only ensures your safety but it also saves energy

18. tissue culture hoods med.cam.ac.uk newvoiceforresearch.blogspot.com

19. tissue culture hoods: how they work ars.usda.gov

20. tissue culture hoods energy consumption 1 – 12.6 kWh/day depending on usage -manufacturer data 0.3 – 0.6 kWh/day per UV lamp -manufacturer data

21. ‘be good in the hood’

22. turn hood COMPLETELY off thelabworldgroup.com prevents UV damage to reagents and staff

23. ‘be good in the hood’ being good in the tc hood not only ensures your safety and prevents unnecessary UV damage to hood contents but it also saves energy

24. ultra-low freezers

25. ultra-low freezers: -80 energy consumption 15 – 20 kWh/day -manufacturer data -validated by monitoring

26. ‘-70 is the new -80’

27. chill up freezers from -80 to -70 save 2-4kW per day

28. DOE ‘-70 is the new -80’

29. clean freezer coils, regularly defrost freezer save 12-25% energy other best practices for ultra-low freezers prolong lifetime of the freezer

30. perform scheduled maintenance on freezers other best practices for ultra-low freezers prolong lifetime of the freezer

31. remove old, unneeded samples other best practices for ultra-low freezers fewer samples stored, fewer freezers are needed

32. transfer samples to other storage paradigms -20 freezers ambient temperature storage containers other best practices for ultra-low freezers fewer samples stored, fewer freezers are needed

33. lasers laserfest.org

34. lasers: types gas lasers solid state

35. lasers: gas energy consumption 15 – 120 kWh/day -manufacturer data

36. exchange gas lasers for solid state lasers could save a minimum of 50% energy -manufacturer data best practices for lasers solid state lasers last longer, lower maintenance costs

37. prolongs lifetime of the laser saves energy best practices for lasers turn off (gas) lasers when not in use, especially over the weekend

38. microscopes

39. mercury-free microscopy

40. mercury/metal halide bulbs mercury-free microscopy

41. mercury/metal halide bulbs 5000 hours/mg Hg 2.5 hours/mg Hg 1 bulb 2000 bulbs mercury-free microscopy

42. recycle used mercury and metal halide bulbs

43. energy consumption illumination: 0.12 – 6 kWh/day -monitored by My Green Lab other best practices for microscopy

44. exchange mercury/metal halide for solid state light sources savings 25 – 90% -monitored by My Green Lab other best practices for microscopy less toxic, cost less to own, in many cases are better for samples

45. cost of ownership of microscope illumination sources MERCURY ARCMETAL HALIDESOLID STATE Replacement bulbs15015n/a Total Bulb Costs$22,500$10,500$0 Total Liquid Light Guide Costs n/a$5,250$0

46. LED comparison with metal halide: stability

47. LED comparison with metal halide: ‘brightness’ metal halide (300hrs) v. sold state

48. LED comparison with metal halide: energy consumption

49. other best practices for microscopy turn off microscope equipment when not in use prolongs lifetime of the equipment saves energy

50. large centrifuges djblabcare.co.uk

51. large centrifuges energy consumption 0.7 – 6 kWh/day -manufacturer data

52. store rotors at 4  C energy savings minimum 10% best practices for large centrifuges saves time

53. turn centrifuge off after use savings minimum 10% best practices for large centrifuges prolongs lifetime

54. heating blocks phenixresearch.com

55. heating blocks energy consumption 0.5 – 12 kWh/day -S-labs

56. best practices for heating blocks turn them off when not in use energy savings minimum 10% prolongs lifetime improves safety

57. autoclaves oh.water.usgs.gov lightlabsusa.com

58. autoclaves: types stand-alone bench-top

59. autoclaves water consumption 90 gallons hot water per cycle 22 gallons cold water for effluent cooling -UCSB

60. autoclaves University of Edinburgh autoclaves accounted for 41% of the total energy usage on campus energy consumption

61. combine loads use a smaller, research –grade autoclave best practices for autoclaves keep autoclave in ‘standby’ rather than ‘on’

62. water tap water distilled water deionized water ultrapure water

63. water tap water distilled water deionized water ultrapure water purified

64. distilled water (ddH 2 O) www.stuart-equipment.com energy and water intense

65. deionized water (DI) pre-treatment reverse osmosis polishing (deionization) UV sterilization, final filtration usually stored 50% efficient

66. ultrapure water: Millipore (MilliQ) goes through a filter 35-50% efficient, larger systems more efficient -UCSB

67. only use what is necessary – keep in mind that what you use is just a fraction of the total water being used recycle filter cartridges through Millipore best practices for water consumption

68. consumables

69. plastics

70. caissonlabs.comlabnetinternational.com

71. plastics waste stream est 12% is recycled every year est 1100 square miles of plastic leave labs -Mt. Baker Bio

72. recycle plastics that can be recycled best practices for plastics utilize vendor recycling programs use biodegradable plastics use only what is needed

73. VWR: TerraCycle for all pipet tip boxes vendor recycling programs Genessee Scientific: pipet tip boxes only from GS Mt. Baker Bio: pipet tip boxes, reagent bottles

74. gloves wikipedia.org

75. gloves waste stream 1000s of pounds – not well documented

76. reuse gloves when possible best practices for gloves glove recycling program through Kimberly-Clark

77. animal bedding drsforstersmith.com petcity.com.au

78. animal bedding waste stream usually sent to a landfill Emory University produces 22 tons/month

79. compost best practices for animal bedding “bedder” animal bedding