1. Chromatography 研習資料 Separation of Pigments In a solvent according To their densities

2. Separate plant pigments

3. Also for Forensics Find type of ink (different color separations) Find impurities in products

4. Taper Strip Cut to a point with scissors

5. Mark a starting line Use pencil (not ink)

6. Chemical Application Add small drops, let dry, add again until dark spot KEEP ABOVE PENCIL LINE

8. Use coin (mechanical) – geranium leaf Use serrated coin Do a few times to make a dark line

9. Be sure liquid is below line Attach paper to paper clip Attach paper clip to stopper

10. Chromatography Animation Paper Chromatography Videos

11. Chromatography Over Time Time Lapse Video (Be patient and give it time to move)

12. Mark bands of colors Lighter pigments will move farther up the paper Heavier pigments will remain near the start

13. Pencil line will mark start point

14. Separation of Ink

15. Calculating R f Compares distance the solvent traveled to the distance the test sample traveled. Rf = distance of test sample distance solvent

16. Measure tops of colors From starting point at bottom to top of each color Distance of solvent

17. Pigments in Plants carotene-yellow orange chlorophyll a-bright green Xanthophylls-yellow chlorophyll b-khaki green

18. More colors phycocyanin = bluish phycoerythrin = reddish

19. Carrier Solution Stored in da hood! Return the waste to the waste container in da hood. Boy-e-e-e-e-e-e-e-e-e-e!

20. Sample Results

21. Click Here for Examples Results of Chromatograph

22. Accessory Pigments fucoxanthin = brown Phycobilin = reds phycocyanin = bluish

23. Conclusion Question #2