2. I. Observation or Introduction to the Problem II. History of Field or Background Information III. Hypothesis or Objectives IV. Methods of Study a. Techniques b. Experimental Design or Protocol V. Results (4-6 slides) VI. Summary (state the findings) VII. Conclusions, Speculations and Plans

3. CHECK OUT THE ROOM IN ADVANCE  SIZE OF ROOM  PODIUM  POINTER  COMPUTER HOOKUP  MICROPHONE  SCREEN

5. felt vs thought or believe incidence vs prevalence phenomenon(na) parameter vs variable constitute vs comprise (consist) effects vs affects

7. #1 Mistake in Science Talks: The Assumption That the Audience Knows What You are Talking About Keep it Simple: Explain!

8. Introduction- Observation Keep it simple: engage the audience Sets up the rationale for study Abstract talks: the first slide reflects title Long talks: Historical basis of problem Current understanding Background information

10.  Must have one and be able to state it Make no assumptions Be specific

11.  Tailor description to audience  Simplify  Explain methods  Explain abbreviations and jargon  Time line must be clear

12. Methods In order to measure isoprostanes, we first collected pig urine

13. Figures and Data Slides Declarative title Point to axes-describe 1 min per slide Speak to the slide Don’t over-interpret data Avoid tables!

14. Common Errors in Data Display and Graphing Indexing/Proportionality Scaling Readability Complexity Credibility

16. After careful study, most investigators have concluded that tables generally stink as a way to portray information to an audience. Summary Conclusion If you want to present a good talk, avoid tables.

17. Proportionality Problems: What is a Doubling? Radius = 1 Area = r 2 Volume = r 3 r = 1 Area = 1 Volume = 3 r = 2 Area = 4 Volume = 8 r = 1.26 Area = 1.6 Volume = 2

19. 100 50 0 mmHg E TIME …. ………. … SBP …. … ………. Ppa Pa Pressure Doubled After Endotoxin

20. 30 20 10 0 Ppa E TIME Ppa Doubles After Endotoxin

21. X 100 0 PRE POST ….....…..….. …... ….. …... …. X Increased after Treatment

22. 100 X 0 PRE POST.. X Increased after Treatment

25. Substance P is a Potent Pressor 80 70 mmHg PRE POST

26. 100 0 mmHg Substance P is a Mild Pressor PRE POST

27. TGFb: Involvement in PPH Pathogenesis Cell proliferation, differentiation Apoptosis Morphogenesis Organogenesis

28. Genetic Mapping Family Studies Chromosome Interval Met A A Met T T G G Val G G Val T T C C Ser T T Ser C C A A Leu C C Leu T T G G Gln C T A A Pro C C C C G G Cys T T G G T T STOP * 1.ESTs, unidentied 2. ESTs, unidentied 3.ESTs, highly similar to patched [Drosophila melanogaster] 4.Phosphofructokinase (PFK) 5. BMPR2 Positional & Functional candidate for PPH 6.ESTs, unidentied 7.Deleted in pancreatic cancer 1 (DPC1) 8.ESTs, unidentied Genes in Interval Computer Search Mutation Detection Candidate Genes Disease Mutation Linkage & Disease Gene Hunting 2000 + HGP

30.  Summary: be concise statement of facts  Conclusions: meaning or lesson speculations  Problems and Plans: what is next what to fix

31. Oh, yes: finally you probably shouldn’t make every slide a different font and color