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Published byHoratio Lang Modified over 9 years ago
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Buffy Coat Method Platelet Poor Plasma Step 1: Whole Blood is collected into a top and bottom pack and is centrifuged (hard spin) to produce platelet poor plasma, red blood cells and a buffy coat Buffy Coat Layer Red Cell Concentrate Red Cell Additive Images courtesy of Canadian Blood Services – Hospital Education Presentation 2004
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Step 2 Extraction Plasma Red blood cell Buffy Coat Buffy Coat Method cont.
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Step 3: Pooled Buffy Coats are first pooled, then sterile docked to the platelet storage bags, followed by centrifugation at low speed Buffy Coat Method cont.
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Buffy Coats sterile docked together Plasma Pooling (Train Method) Buffy Coat Method cont. Platelet storage bag sterile docked to the Buff Coat “train”
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Buffy Coat Pool is loaded into buffy coat bag Supernatant (PRP) pressed through LR filter into storage bag Buffy Coat Method cont
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Pooled Platelet Concentrate, LR is Similar in physical size & dosage to an apheresis platelet concentrate Buffy Coat Method cont.
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