1. Susan Hollingsworth Aaron Kaiser Biology 110 Lab # smh092404

2. Purpose:  To observe multiple environments that harbor bacterial growth and determine which environment under investigation is most conducive to bacterial growth  Environments under investigation: airsoil washed/unwashed hands door handle of men’s bathroom raw chicken pond water

3. Hypothesis:  Pond Water  largest and most diverse population of bacteria due to a vast array of living and decomposing organisms that are vulnerable to bacterial colonization  aquatic medium that receives sunlight (some bacteria are photosynthetic)  exposed to air which transports bacterial spores.

4. Materials:  7 sterile Petri dishes with nutrient agar  Wax pencil  9 bacterial inoculating loops  Sterile cotton swab  Piece of raw chicken  Soil sample  Pond water sample  Hand soap  Sterile water  Parafilm strips  Light microscope  Dissecting microscope  incubator

5. Method: 1.Label each Petri dish 2.“Air” sample 3.Use Streak Plate method to apply sample (a) (b)(c) Figure 1. Isolating bacterial colonies using the “Streak Plate” method.

6. Data Interpretations: 4.Unwashed/Washed hand sample 5.Recover dish labeled “air.” 6.Wrap cultures with Parafilm and incubate upside down for 1 week. The Petri dishes will be analyzed by using colony morphology characteristics to see which one has the most species of bacteria. This will be accomplished by identifying and tabulating the number of different colonies present in each sample.

7. ColonySizeShapeMarginSurfaceColor a.6mmIrregularLobateWrinkled Milky white b.4mmRoundWavySmooth White center, clear surrounding c.13mmIrregularLobateSmooth White center, milky white surrounding d.5mmIrregularWavySmooth Yellow, gold, clear surrounding e.9.5mmIrregularWavy Smooth, contoured edges Tan center, white ring, clear ring Table 1. Bacteria colony morphology: Air Fig. 2 Bacteria present in Air environemnt

8. ColonySizeShapeMarginSurfaceColor a.1mmRoundLobateWrinkled Creamy white to slightly yellow b.5mmRoundSmoothSmoothwhite ColonySizeShapeMargin Surfac e Colora.10mm No defined shape No defined margin WrinkledClear b. Less than 1mm IrregularLobateWrinkled Cream white Table 2. Bacteria colony morphology: Chicken Table 3. Bacteria colony morphology: Soil Fig 3. Bacteria found on Chicken environment Fig 4. Bacteria found on soil environment

9. ColonyShapeSizeMarginSurfaceColor a.3mmRoundLobateSmooth Creamy white b.4mmRoundLobateSmoothClear c.PunctiformRoundSmoothSmoothwhite Table 5. Bacteria colony morphology: washed hand Fig 5. Bacteria found on washed and unwashed hand environments

10. ColonySizeShapeMarginSurfaceColor a.8mmIrregularSmoothSmoothYellow b.8mmRoundLobateSmooth Clear to creamy white c.3mmRoundSmoothConcentricWhite d.2mmRoundLobateSmooth Clear to off white e.3mmRoundLobate Wrinkled and smooth Clear f. Punctiform on e. RoundSmoothSmooth Slightly white to clear g.7mmIrregularWavySmooth Creamy white Table 4. Bacteria colony morphology: unwashed hand

11. ColonySizeShapeMarginSurfaceColor a.4mmRoundLobateSmooth Tan to white b.1mmRoundSmoothSmoothOrange c.5mmRoundSmoothSmooth Clear to off white d.30mmIrregularLobateSmoothtrasparent Table 6. Bacteria colony morphology: pond water Fig 7. Bacteria found on pond water environment

12. ColonySizeShapeMarginSurfaceColor a.3mmRoundLobateSmoothClear b.3mmRoundLobateSmoothOrange c.10mmRoundSmoothSmoothyellow d.10mmRoundSmoothSmoothwhite e.6mmRoundWavyMucoidalwhite Table 7. Bacteria colony morphology: Men’s bathroom door Fig 8. Bacteria found on bathroom door environment

13. Side Study: Effect of Antibiotics on E. coli growth AntibioticSensitivity Chloramphenicol- 30mg NS Penicillin-10mgNS Nalidixic Acid-30mg VS* Norobiocin-30mgNS Streptomycin-10mgVS Erythromycin-15mgS Tetracycline-30mgVS Kanamycin-30mgVS Fig 9. E coli growth among antibiotics