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ENDURO Horizontal Electrophoresis Systems. Casting a Gel Attach a buffer dam to one side of gel tray Attach the other buffer dam to the other side of.

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Presentation on theme: "ENDURO Horizontal Electrophoresis Systems. Casting a Gel Attach a buffer dam to one side of gel tray Attach the other buffer dam to the other side of."— Presentation transcript:

1 ENDURO Horizontal Electrophoresis Systems

2 Casting a Gel Attach a buffer dam to one side of gel tray Attach the other buffer dam to the other side of gel tray Place gel tray on a flat surface

3 Casting a gel Mix agarose buffer solution and boil Cool agarose solution to below 60ºC Pour onto gel tray Let solution cool for ½ hour Gently remove combs

4 Running the Gel  Remove the casting gates  Place the gel tray into the gel box  Overlay it with buffer  Connect the lid & attach to a power supply

5 Staining the Gel 1.Remove gel from gel tray 2.Place into a container with stain 3.Destain the gel with buffer or water

6 Features Heavy duty molded chambers Removable electrodes Multichannel pipette compatible combs Domed lid to prevent condensation

7 Heavy duty molded construction Prevents leaking problems

8 Changing the electrode Turn unit over & remove screw Remove old electrode Insert new electrode & secure with nylon screw

9 Multichannel Combs A large number of comb are compatible with multichannel pipettes

10 Domed Lid Prevents condensation from falling on the gel Recaptures condensation in reservoirs


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