1. Presented by: Ms. N. Mapholi (MSc in Genetics)
The application of DNA technology in Animal Forensic and Parentage Determination
Presented by: Ms. N. Mapholi
(MSc in Genetics)

2. ARC-API: Animal Genetics Lab
Animal forensics in livestock and game poaching
Cattle and horse DNA profiling and Parentage Determination
Species Identification on meat and process meat
Genetic Characterization
Genetic Defects tests
LIDCAT system
Gene mapping and linkage studies

3. Technologies Genotyping Real time PCR Microsatellites marker panels
Species specifics panels
Real time PCR
Primer designed kits
SNPs assays

4. FORENSIC WORK Stock theft and poaching Microsatellites markers
Link the crime scene and the suspect DNA evidences obtained
Parentage dispute cases
Different marker panels
Bovine, Horse, Ovine, Goat, Porcine, Buffalo, Impala, Kudu, Wildebeest, Eland, Sable, Rhino and Blesbok

5. Chain of custody Herd of sheep Crime scene New case 3130xL Gene scan
Thermal cycler
DNA extract
DNA Samples

6. Results Interpretation
Court
Results Interpretation
DNA Report
Jail
Judgement

7. Parentage dispute DNA samples from both parents and calf
Alleged mothers and dispute calf
It is important to take note that in the absence of either parent (bull or cow) paternity cannot be proven.
Only when both parents are analyzed it can be proven beyond doubt that the parents are the real biological parents of the offspring in question

8. Parentage dispute Inclusion/ exclusion as possible mothers Cow 1 Cow 2
Calf 1?

9. … Procedure of sampling hair Hair Samples PCR tubes PCR tray
Genetic analyzer
PCR machine

10. DNA profiling
Service rendered to the breed societies and stud breeders for bovine and horses
DNA profiling certificates are issued
Parentage determination for breeding and selection purpose
Buffalo DNA profiling – this is new and still building the database

11. Real Time PCR DNA based technology
Species identification on meat and processed meat products
biltong, wors, patties, sausages, mince meat, Vienna
Species specific primer designed kits
SNP Assays for genetic defects tests in bovine and porcine
Dexter chondodysplasia and Malignant hyperthermia

12. Genetic characterization
Most of livestock species haven been characterized in our lab using microsatellites markers
Several multiplexes have been developed and optimized in our lab

13. LIDCAT DATABASE
Lidcat package – for tail hair collection

14. Information needed on LIDCAT system
Animal ID (ear tag number)
Species (cattle)
Breed (Nguni)
Gender (female or male)
Name of the owner (Olivia Mapholi)
Postal address of owner
Contact numbers

15. Contact details Ms. Olivia Mapholi Tel : 012 672 9027/ 9008/ 9187
Fax:

16. Thank you
Any questions?