1. Figure 1 HuR Tis11b HPRT VEGF 0 1 2 3 4 5Time (h) A 462 bp (VEGF 165 ) 332 bp (VEGF 121 ) 354 bp 111 bp 305 bp

2. Figure 2 Cytoplasm (20 µg)Nuclei (5 µg) HuR  -tubulin 0 0.5 1 2 4 6 Lamin A/C Time (h) 0 0.5 1 2 4 6 Tis11b C 0 1 2 3 4 5 6 HuR  -tubulin Total cell extracts Tis11b Time (h) AB 0.10 0.15 0.20 0.25 0.30 0.35 0.40 0.45 0.50 01234567 Time (h) Tis11b/tubulin or HuR/tubulin Tis11b HuR

3. Figure 3 10000 15000 20000 25000 30000 35000 012345 Time (h) HuR protein levels (arbitrary density units) Cytoplasm Nuclei B Cytoplasm Nuclei HuR IgG light chain HuR IgG light chain Time (h) 0 1 2 3 4 Time (h) 0 1 2 3 4 pp32 IgG light chain A

4. Figure 4 Time (h)0 0.5 1 2 3 4 B VEGF HPRT - LMB+ LMB VEGF 165 VEGF 121 C 0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 01234 Time (h) VEGF/HPRT (fold induction) ACTH ACTH+LMB A Nuclei Time (h) 0 0.5 1 2 3 4 - LMB+ LMB Cytoplasm HuR  -tubulin Lamin A/C 0 0.5 1 2 3 4Time (h) Lamin A/C HuR  -tubulin

5. Figure 5 Time (h) 0 1 2 3 4 5 6 9 Negative control siRNA + siRNA HuR HPRT VEGF HuR 0 1 2 3 4 5 6 9 A VEGF 165 VEGF 121 C 0 25 50 75 100 125 150 175 200 225 250 012345678910 Time (h) VEGF/HPRT (% of control) ACTH - siRNA ACTH + siRNA B  -tubulin + siRNA HuR Time (h) 0 0.5 1 2 3 4 5 6 HuR 0 0.5 1 2 3 4 5 6 Negative control siRNA

6. Figure 6 C 0 0.05 0.1 - - 0.1 0.1 0 - - 0.2 1 0.2 1 HuR (ng) Tis11b (ng) HuR Tis11b 0 20 40 60 80 100 120 140 160 180 Firefly Luc/Renilla (% of Control) + +++ + ** * *** A 0 0.05 0.10 0.15 0.20 0.25 0.30 0.35 00.10.515 Tis11b (ng) Firefly Luc/Renilla Luc ** *** 0.40 B 0 0.05 0.10 0.15 0.20 0.25 0.30 0.35 0.40 0.45 0.50 00.050.10.51 HuR (ng) Firefly Luc/Renilla Luc ** 0.55 0 0.1 0.1 0.1 - - 0 - 0.2 1 0.2 1 HuR (ng) Tis11b (ng) 18S Luciferase D

7. Figure 7 A FL 5’ 3’ StuIStyI NsiI AflII SmaI 1 198 862 1081 1255 1828 2201 µg HuR 0 0 1 2 5 µg Tis11b 0 20 2 4 6 1 ns GST-HuR Tis11b 3’UTR FL * RNA 64.2 kDa 37.1 kDa 25.9 kDa B 1 2 3 4 5 6 7 8 9 0 25 50 75 100 125 0123456 HuR or Tis11b (µg) % of binding Tis11b HuR C