1. Teachable Tidbit: Mobility of membrane lipids and proteins I.Pre-assessment group discussion II.Lecture/discussion/ Experimental examples III.Post unit assessment: problem set and quiz

2. The fluid mosaic model for biological membranes: A lipid bilayer within which integral membrane proteins are inserted into and through the bilayer, but unless restrained, free to move within the plane of the membrane.

3. Topic/unit objective: Understanding the nature of and functional significance of the dynamics of lipids and proteins in biological membranes Pre-unit assessment goal: How would you demonstrate the mobility of lipids and/or proteins in biological membranes? CRAZY IDEAS WELCOME: Fluid Mosaic Model for Biological Membranes

4. I.Modes of lipid mobility; factors that affect mobility II.Topographic organization of biological membrane proteins III.Lateral mobility of integral membrane proteins I.Frye and Edidin experiment demonstrating lateral mobility of cell surface proteins in mouse/human heterokaryons II.Antibody “capping” of cell surface membrane proteins III.Kinetic analysis of lateral membrane protein mobility: FRAP and FLIP analysis IV.Modes of regulating membrane protein mobility

5. Fluorescence Recovery after Photobleaching (FRAP)

7. Fluorescence recovery after photobleaching

8. Nuclear lamina on inner surface of frog egg nucleus. Lamin dimers