1. For Internal Use Only

2. Dot Plot Page a.Histograms b.Scatter Plots c.Flow Rate Plots d.Hemoglobin Plots Data Page a.General run information – Fields 1-10 and 54 b.CBC5R Information – Fields Calibration Factors – Fields 16-22 c.Diagnostic Values – Fields 23-34 and 51-53 d.Patient Results – Fields 35-49 e.Flags –Field 50 For Internal Use Only

3. FSL HISTOGRAM FSH HISTOGRAM EXT HISTOGRAM RAS HISTOGRAM TOF HISTOGRAM RED HISTOGRAMS For Internal Use Only

4. EXT RAS EXT vs RAS RED SCATTER PLOTS FSH FSL FSL vs FSH EXT vs TOF EXT TOF For Internal Use Only

5. WHITE HISTOGRAMS For Internal Use Only FSL HISTOGRAMFSH HISTOGRAM EXT HISTOGRAM RAS HISTOGRAM TOF HISTOGRAM

6. WHITE SCATTER PLOTS For Internal Use Only EXT vs RAS EXT RAS TOF vs FSH TOF FSH TOF vs RAS int. TOF RAS int.

7. FLOW RATE PLOTS For Internal Use Only RED WHITE

8. HGB PLOTS For Internal Use Only RED WHITE

9. For Internal Use Only

10. 1.Compare the two Hemoglobin Values to check dilutions 2.EXT vs RAS for Red Run 3.EXT vs RAS for White Run 4.TOF vs FSH for White Run THE BASICS OF DOT PLOT ANALYSIS For Internal Use Only

11. HGB value from white run HGB value from red run Comparing the two HGB values to check dilutions. 1.The two hemoglobin values are obtained from separate dilutions 2.The Red HGB is measured with the blue LED while the HGB is still inside the RBCs 3.The White HGB is measured with the green LED after the HGB has been released from the RBCs – this is the reported value 4.The two values are usually within a few g/dL of each other. For Internal Use Only

12. White HGB = 13.51 Red HGB = 12.43 HGB values are close Conclusion: Dilutions were appropriate For Internal Use Only

13. EXT - RAS for the Red Run For Internal Use Only

14. EXT - RAS for the Red Run RED BLOOD CELLS For Internal Use Only

15. EXT - RAS for the Red Run PLATELETS For Internal Use Only

16. EXT - RAS for the Red Run RBC FRAGMENTS For Internal Use Only

17. EXT - RAS for the Red Run RETICULOCYTES For Internal Use Only

18. EXT - RAS for the Red Run DOUBLETS For Internal Use Only

19. TOF-FSH for the White Run

20. For Internal Use Only TOF-FSH for the White Run

21. For Internal Use Only TOF-FSH for the White Run Unlysed Red Blood Cells (uRBCs)

22. For Internal Use Only EXT-RAS for the White Run

23. For Internal Use Only EXT-RAS for the White Run

24. For Internal Use Only EXT-RAS for the White Run Qualibeads

25. For Internal Use Only EXT-RAS for the White Run Lymphocytes

26. For Internal Use Only EXT-RAS for the White Run Monocytes

27. For Internal Use Only EXT-RAS for the White Run Neutrophils

28. For Internal Use Only EXT-RAS for the White Run Eosinophils

29. For Internal Use Only EXT-RAS for the White Run Basophils

30. For Internal Use Only EXT-RAS for the White Run R7 – Residual WBCs from Red Run

31. For Internal Use Only EXT-RAS for the White Run R8 – Platelets, Aggregated Platelets, and sometimes Decayed Neutrophils

32. For Internal Use Only Differences Between Species 1.Appearance of RBC population on EXT-RAS 2.Location of Eosinophils on scatter plots 3.General size of the Unlysed RBC population 4.Tendency for platelets to aggregate during the blood draw

33. Appearance of RBC Population on EXT - RAS Canine Feline Equine For Internal Use Only

34. Location of Eosinophils on Scatter Plots Canine Left of Neuts on EXT-RAS Feline Right of Neuts on EXT - RAS Equine Right on Neuts on TOF - FSH For Internal Use Only

35. General Size of Unlysed RBC Population Canine Few to Moderate Numbers uRBC Feline Large Numbers uRBC Equine No uRBC to Few uRBC For Internal Use Only

36. Tendency for Platelets to Aggregate During the Blood Draw Equine – Platelets will Rarely Aggregate Canine – Platelets will Sometimes Aggregate Feline – Platelets will Frequently Aggregate For Internal Use Only

37. High Platelet Aggregates vs No Platelet Aggregates Platelet Aggregates No Platelet Aggregates

38. Questions? Practice Analyzing Dotplots