1. Material Properties Repository ImageAnalyzer GUI (MATLAB)
Microstructure Image Analyzer
Object attributes:
Area
Centroid
Nearest neighbor dist.
Major axis length
Minor axis length
Orientation
Diameter
Voids, grains, particles
Model Requirements:
Volume/area fraction
Number density
Volume/area
Size distribution
Web
Services
Material Properties Repository
Image analysis
wrapper (MATLAB)
Output constants to:
- plasticity-damage model
- multistage fatigue model
ImageAnalyzer
(MATLAB)
ImageAnalyzer GUI (MATLAB)
T.N. Williams
R.L. Carino
T. Haupt

2. Image Analysis Sequence
Load an image file
Select the image component (R, G, B, gray) that best highlights the objects of interest
Establish the length of the scale bar in microns and pixels
Exclude image regions or pixels from the analysis
Run threshold analysis, or histogram equalization
Visually verify analysis results
Store or transfer results

3. 1. Load an image file
…\AZ31\Microscopy\Untested\
20x-TT-Untested.jpg

4. 2. Select image component that best highlights the objects of interest

5. 3. Establish image scale
How many pixels
in 50 microns?

6. 3.a. Select the scale area as “Region of Interest” (ROI)
Click for corners of a polygon
to enclose the scale bar.
Double-click to close polygon.

7. 3.b. Enter length of scale bar in microns.
But how many pixels?

8. 3.c.i. Determine length of scale bar in pixels
Click-drag pointer
from here …
… to here
… and remember this value.

9. 3.c.ii. Enter length of scale bar in pixels

10. 3.c.iii. Or, use “Guess pixels…” to determine the length of scale bar in pixels
Click on the scale bar, then
press the “Enter” key.
Enter -5/+5 the selected
pixel intensity for Min/Max.

11. 3.c.iv. Confirm the determination of the length of scale bar in pixels
Check if scale bar was correctly determined.

12. 3.d. Clear the ROI after establishing the image scale

13. 4. Exclude pixels from analysis
Select the annotation as
A “Region Of Interest”

14. 4.a. Select a representative pixel to exclude
Click on a representative
pixel, then press the
“Enter” key.
Enter -5/+5 the selected
pixel intensity for Min/Max.

15. 4.b. Select a replacement color
Click on the replacement color, then press the
“Enter” key.

16. 4.c. Confirm exclusion of pixels

17. 4.d. Clear the ROI after exclusion of pixels

18. 4.e. Other functions to exclude pixels from analysis

19. 5.a. Threshold analysis Enter minimum area for objects, in microns
OR in pixels
Enter range of pixel intensities for objects

20. 5.b. Histogram equalization analysis
Enter minimum
area for objects,
in microns
OR in pixels
Enter no. of gray levels
Enter range of gray levels for objects

21. 6.a. Display original AND marked image
Right-click on image

22. 6.b. Verify that the objects of interest are included; iterate analysis as necessary.

23. 6.c. Display histograms of object properties.
Enter range of values for property
Enter no. of histogram bins

24. 6.d. Sample histogram
Left-click a point on “Cumulative frequency” curve …
“79.93% of the objects have areas less than 50.33μm2”
… then on an empty space

25. 7.a. Store or transfer results
“Transfer” is enabled if ImageAnalyzer is invoked by DMG or MSF

26. 7.b. File outputs Aggregate statistics
Details for each object, and histograms
Restart file

27. Feature: Multiple images (with same scale)
Click File | Select for analysis… after loading several images.