Download presentation
Presentation is loading. Please wait.
1
PowerPoint Presentation Materials to accompany
Genetics: Analysis and Principles Robert J. Brooker CHAPTER 4 EXTENSIONS OF MENDELIAN INHERITANCE Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
2
Symbols for Alleles Dominant alleles are usually indicated either by an italic uppercase letter (D) or by a an italic letter or group of letters followed by a superscript + (Wr+). Recessive alleles are usually indicated either by an italic lowercase letter (d) or by an italic letter or group of letters (Wr) without the +. If no dominance exists, italic uppercase letters and superscripts are used to denote alternative alleles (R1, R2; CW, CR).
3
Morgan’s Experiment The chromosome theory of inheritance was confirmed through studies carried out by Thomas Hunt Morgan Morgan tried to induce mutations into the fruit fly Drosophila melanogaster Treatments included Rearing in the dark X-rays Radium 3-82 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
4
After 2 years, Morgan finally obtained an interesting result
A male fruit fly with white eyes rather than the normal red eyes Morgan reasoned that this white eyed male must have arisen from a new mutation that converted a red-eyed allele into a white-eyed allele Morgan followed Mendel’s approach in studying the inheritance of this white-eyed trait He made crosses then analyzed their outcome quantitatively 3-83 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
5
Morgan’s first mutant
6
Testing the Hypothesis
A quantitative analysis of genetic crosses may reveal the inheritance pattern for the white eye allele Testing the Hypothesis Refer to Figure 3.19 3-84 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
7
XW+Y male offspring and XW+XW female offspring, both with red eyes
Fig (TE Art) Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Experimental level Conceptual level 1. Cross the white-eyed male to red-eyed females. XWY x XW+X+ x 2. Record the results of the F1generation. This involves noting the eye color and sexes of several thousand flies. XW+Y male offspring and XW+XW female offspring, both with red eyes x XW+Y x XW+XW F1 generation 3. Cross F1 offspring with each other to obtain F2 offspring. Also record the eyecolor and sex of the F2 offspring. 1 XW+Y : 1 XWY : 1 XW+XW+ : 1 XW+XW 1 red-eyed male : 1 white-eyed male : 2 red-eyed females F2 generation 4. In a separate experiment, perform a testcross between a white-eyed male and a red-eyed female from the F1generation. Record the results. XWY x XW+XW x From F1 generation 1 XW+Y : 1 XWY : 1 XW+XW+ : 1 XW+XW 1 red-eyed male : 1 white-eyed male : 1 red-eyed females : 1 white-eyed female
8
The Data Cross Results Test Cross Results 3-86
Original white eyed-male to red-eyed females F1 generation: All red-eyed flies F1 male to F1 female F2 generation: ,459 red-eyed females 1,011 red-eyed males 0 white eyed-females 782 white-eyed males Test Cross Results White-eyed male to F1 female 129 red-eyed females 132 red-eyed males 88 white eyed-females 86 white-eyed males 3-86 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
9
Interpreting the Data The first cross yielded NO white-eyed females in the F2 generation These results indicate that the eye color alleles are located on the X chromosome Genes that are physically located on the X chromosome are called X-linked genes or X-linked alleles 3-87 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
10
A Punnett square predicts the absence of white-eyed females in the F2 generation
F1 male is Xw+Y F1 female is Xw+Xw + 3-88 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
11
The testcross resulted in red-eyed females and males, and white-eyed females and males, in approximately equal numbers This is consistent with an X-linked pattern of inheritance Male is XwY F1 female is Xw+Xw 3-90 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
12
Reciprocal crosses Crosses between different strains in which the sexes are reversed These crosses reveal whether a trait is carried on a sex chromosome or an autosome X-linked traits do not behave identically in reciprocal crosses 3-92 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
13
Consider the following two crosses:
Male is Xw+Y Female is XwXw Male is XwY Female is Xw+Xw+ 3-93 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
14
When comparing the two Punnett squares, the outcomes of the reciprocal cross did not yield the same results the male transmits X-linked genes only to his daughters the female transmits X-linked genes to all her children This explains why X-linked traits do not behave equally in reciprocal crosses 3-94 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
15
Extensions of Mendelian Inheritance
Mendelian inheritance describes inheritance patterns that obey two laws Law of segregation Law of independent assortment Simple Mendelian inheritance involves A single gene with two different alleles Alleles display a simple dominant/recessive relationship Extensions of Mendelian inheritance are more complex and may involve multiple alleles and/or multiple genes 4-2 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
16
4-5 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
17
Prevalent alleles in a population are termed wild-type alleles
These typically encode proteins that Function normally Are made in the right amounts Alleles that have been altered by mutation are termed mutant alleles These tend to be rare in natural populations They are likely to cause a reduction in the amount or function of the encoded protein Such mutant alleles are often inherited in a recessive fashion 4-6 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
18
Consider, for example, the traits that Mendel studied
Wild-type (dominant) allele Mutant (recessive) allele Purple flowers White flowers Axial flowers Terminal flowers Yellow seeds Green seeds Round seeds Wrinkled seeds Smooth pods Constricted pods Green pods Yellow pods Tall plants Dwarf plants Another example is from Drosophila Wild-type (dominant) allele Mutant (recessive) allele Red eyes White eyes Normal wings Miniature wings 4-7 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
19
Genetic diseases are caused by mutant alleles
In many human genetic diseases , the recessive allele contains a mutation This prevents the allele from producing a fully functional protein 4-8 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
20
There are two possible explanations
In a simple dominant/recessive relationship, the recessive allele does not affect the phenotype of the heterozygote So how can the wild-type phenotype of the heterozygote be explained? There are two possible explanations 1. 50% of the normal protein is enough to accomplish the protein’s cellular function Refer to Figure 4.1 2. The heterozygote may actually produce more than 50% of the functional protein The normal gene is “up-regulated” to compensate for the lack of function of the defective allele 4-9 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
21
Complete Dominance 4-10 Figure 4.1
Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
22
Lethal Alleles Essential genes are those that are absolutely required for survival The absence of their protein product leads to a lethal phenotype It is estimated that about 1/3 of all genes are essential for survival Nonessential genes are those not absolutely required for survival A lethal allele is one that has the potential to cause the death of an organism These alleles are typically the result of mutations in essential genes They are usually inherited in a recessive manner 4-11 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
23
Many lethal alleles prevent cell division
These will kill an organism at an early age Maintained in populations by heterozygotes Some lethal allele exert their effect later in life Huntington disease (dominant allele) Characterized by progressive degeneration of the nervous system, dementia and early death Maintained in populations by late onset (age 30 to 50) Conditional lethal alleles may kill an organism only when certain environmental conditions prevail Temperature-sensitive (ts) lethals A developing Drosophila larva may be killed at 30 C But it will survive if grown at 22 C 4-12 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
24
An example is the “creeper” allele in chicken
A lethal allele may produce ratios that seemingly deviate from Mendelian ratios An example is the “creeper” allele in chicken Creepers have shortened legs and must creep along Creeper chicken are heterozygous Scot’s Dumpy 4-13
25
Creeper is a dominant allele
Creeper X Normal Creeper X Creeper 1 creeper : 1 normal 1 normal : 2 creeper Creeper is lethal in the homozygous state Creeper is a dominant allele 4-14 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
26
Figure 4-4 Copyright © 2006 Pearson Prentice Hall, Inc.
Figure 4-4 Inheritance patterns in three crosses involving the normal wild-type agouti allele (A) and the mutant yellow allele in the mouse. Note that the mutant allele behaves dominantly to the normal allele in controlling coat color, but it also behaves as a homozygous recessive lethal allele. The genotype does not survive. Figure Copyright © 2006 Pearson Prentice Hall, Inc.
27
Incomplete Dominance In incomplete dominance the heterozygote exhibits a phenotype that is intermediate between the corresponding homozygotes Example: Flower color in the four o’clock plant Two alleles CR = wild-type allele for red flower color CW = allele for white flower color 4-15 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
28
CO 4
29
1:2:1 phenotypic ratio NOT the 3:1 ratio observed in simple Mendelian inheritance
In this case, 50% of the CR protein is not sufficient to produce the red phenotype Figure 4.2 4-16 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
30
Incomplete Dominance Whether a trait is dominant or incompletely dominant may depend on how closely the trait is examined Take, for example, the characteristic of pea shape Mendel visually concluded that RR and Rr genotypes produced round peas rr genotypes produced wrinkled peas However, a microscopic examination of round peas reveals that not all round peas are “created equal” Refer to Figure 4.3 4-17 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
31
Figure 4.3 4-18 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
32
Multiple Alleles Many genes have multiple alleles
Three or more different alleles May display a hierarchy of dominance May display codominance 4-19 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
33
An interesting example is coat color in rabbits
Four different alleles C (full coat color) cch (chinchilla pattern of coat color) Partial defect in pigmentation ch (himalayan pattern of coat color) Pigmentation in only certain parts of the body c (albino) Lack of pigmentation The dominance hierarchy is as follows: C > cch > ch > c Figure 4.4 illustrates the relationship between phenotype and genotype 4-20 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
34
Fig. 4.4
35
The himalayan pattern of coat color is an example of a temperature-sensitive conditional allele
The enzyme encoded by this gene is functional only at low temperatures Therefore, dark fur will only occur in cooler areas of the body This is also the case in the Siamese pattern of coat color in cats Refer to Figures 4.4c and 4.5 4-21 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
36
The ABO blood group provides another example of multiple alleles
It is determined by the type of antigen present on the surface of red blood cells Antigens are substances that are recognized by antibodies produced by the immune system As shown in Table 4.3, there are three different types of antigens found on red blood Antigen A, which is controlled by allele IA Antigen B, which is controlled by allele IB Antigen O, which is controlled by allele i 4-22 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
37
Allele i is recessive to both IA and IB
Alleles IA and IB are codominant They are both expressed in a heterozygous individual N-acetyl- galactosamine B 4-23 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
38
A fourth can be added by the enzyme glycosyl transferase
The carbohydrate tree on the surface of RBCs is composed of three sugars A fourth can be added by the enzyme glycosyl transferase The i allele encodes a defective enzyme The carbohydrate tree is unchanged IA encodes a form of the enzyme that can add N-acetylgalactosamine to the carbohydrate tree IB encodes a form of the enzyme that can add galactose to the carbohydrate tree Thus, the A and B antigens are different enough to be recognized by different antibodies 4-24 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
39
For safe blood transfusions to occur, the donor’s blood must be an appropriate match with the recipient’s blood For example, if a type O individual received blood from a type A, type B or type AB blood Antibodies in the recipient blood will react with antigens in the donated blood cells This causes the donated blood to agglutinate A life-threatening situation may result because of clogging of blood vessels 4-25 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
40
Table 4-1 Copyright © 2006 Pearson Prentice Hall, Inc.
Table 4.1 Potential Phenotypes in the Offspring of Parents with All Possible ABO Blood Group Combinations, Assuming Heterozygosity Whenever Possible Table Copyright © 2006 Pearson Prentice Hall, Inc.
41
Overdominance Overdominance is the phenomenon in which a heterozygote is more vigorous than both of the corresponding homozygotes It is also called heterozygote advantage Example = Sickle-cell anemia Autosomal recessive disorder Affected individuals produce abnormal form of hemoglobin Two alleles HbA Encodes the normal hemoglobin, hemoglobin A HbS Encodes the abnormal hemoglobin, hemoglobin S 4-34 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
42
Fig. 4.9
43
This has two major ramifications
HbSHbS individuals have red blood cells that deform into a sickle shape under conditions of low oxygen tension Refer to Figure 4.9 This has two major ramifications 1. Sickling phenomenon greatly shortens the life span of the red blood cells Anemia results 2. Odd-shaped cells clump Partial or complete blocks in capillary circulation Thus, affected individuals tend to have a shorter life span than unaffected ones 4-35 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
44
The sickle cell allele has been found at a fairly high frequency in parts of Africa where malaria is found How come? Frequencies of the sickle-cell allele 0–2.5% 2.5–5.0% 5.0–7.5% 7.5–10.0% 10.0–12.5% >12.5% Distribution of malaria caused by Plasmodium falciparum (a protozoan)
45
Malaria is caused by a protozoan, Plasmodium
This parasite infects red blood cells Red blood cells of heterozygotes, are likely to rupture when infected by Pasmodium sp. This prevents the propagation of the parasite Therefore, HbAHbS individuals are “better” than HbSHbS, because they do not suffer from sickle cell anemia HbAHbA, because they are more resistant to malaria 4-36 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
46
3. Variation in functional activity
At the molecular level, overdominance is due to two alleles that produce slightly different proteins How can these two protein variants produce a favorable phenotype in the heterozygote? 1. Disease resistance 2. Homodimer formation 3. Variation in functional activity 4-37 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
47
(a) Disease resistance
Fig. 4.10(TE Art) Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Pathogen can successfully propagate Pathogen cannot successfully propagate A1A1 A1A2 Normal homozygote (sensitive to infection) Heterozygote (resistant to infection (a) Disease resistance A1 A1 A2 A2 A1 A2 (b) Homodimer formation E1 E2 27o-32oC 30o-37oC (optimum temperature range) (optimum temperature range) (c) Variation in functional activity
48
Dominance Patterns Caused by Heterodimerization
Activity A1:A1 + A1:A2 - ++ A2:A2 Complete Dominance WT mut Over-dominance Heterozygote>homozygote A1= WT; A2 = mutant
49
Two different highly inbred strains are crossed
Overdominance is related to a common mating strategy used by animal and plant breeders Two different highly inbred strains are crossed The hybrids may display traits superior to both parents This phenomenon is termed hybrid vigor or heterosis Heterosis is used to improve quantitative traits such as size, weight and growth rate 4-41 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
50
Incomplete Penetrance
In some instances, a dominant allele is not expressed in a heterozygote individual Example = Polydactyly Autosomal dominant trait Affected individuals have additional fingers and/or toes A single copy of the polydactyly allele is usually sufficient to cause this condition In some cases, however, individuals carry the dominant allele but do not exhibit the trait 4-42 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
51
Fig. 4.12
52
Figure 4.11 Inherited the polydactyly allele from his mother and passed it on to a daughter and son Does not exhibit the trait himself even though he is a heterozygote 4-43 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
53
Incomplete Penetrance
The term indicates that a dominant allele does not always “penetrate” into the phenotype of the individual The measure of penetrance is described at the population level If 60% of heterozygotes carrying a dominant allele exhibit the trait allele, the trait is 60% penetrant Note: In any particular individual, the trait is either penetrant or not 4-44 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
54
Expressivity Expressivity is the degree to which a trait is expressed
In the case of polydactyly, the number of digits can vary A person with several extra digits has high expressivity of this trait A person with a single extra digit has low expressivity 4-45 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
55
Expressivity of eyeless
56
The molecular explanation of expressivity and incomplete penetrance may not always be understood
In most cases, the range of phenotypes is thought to be due to influences of the Environment and/or Other genes 4-46 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
57
Environment Environmental conditions may have a great impact on the phenotype of the individual Example 1 Snapdragon flower color vs. Temperature and degree of sunlight Figure 4.13 4-47 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
58
Environment Example 2 = Phenylketonuria
Autosomal recessive disorder in humans Caused by a defect in the gene that encodes the enzyme phenylalanine hydroxylase Converts phenylalanine to tyrosine Affected individuals cannot metabolize phenylalanine Phenylalanine will thus accumulate It ultimately causes a number of detrimental effects Mental retardation, for example 4-48 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
59
Environment Example 2 = Phenylketonuria
Newborns are now routinely screened for PKU Individuals with the disease are put on a strict dietary regimen Their diet is essentially phenylalanine-free These individuals tend to develop normally Refer to Figure 1.10 Thus the PKU test prevents a great deal of human suffering Furthermore, it is cost-effective 4-49 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
60
Sex and Traits The inheritance pattern of certain traits is governed by the sex of the individual These traits are of two main types Sex-influenced Sex-limited 4-50 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
61
Sex-influenced Traits
Traits where an allele is dominant in one sex but recessive in the opposite sex Thus, sex influence is a phenomenon of heterozygotes 4-51 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
62
Sex-influenced Traits
Example: Pattern baldness in humans Caused by an autosomal gene Heterozygotes: Allele B behaves as dominant in males, but recessive in females Genotype Phenotype in Females in Males BB bald Bb nonbald bb 4-52 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
63
Sex-influenced Traits
Pattern baldness appears to be related to the level of male sex hormones In females, a rare tumor of the adrenal gland can cause the secretion of large amounts of male sex hormones If this case a heterozygous Bb female will become bald When the tumor is removed surgically, her hair returns to its normal condition 4-53 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
64
Bald fathers can pass the trait to their sons
The autosomal nature of pattern baldness has been revealed by analysis of human pedigrees Refer to Figure 4.15 Figure 4.15 Bald fathers can pass the trait to their sons 4-54 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
65
Sex-limited Traits Traits that occur in only one of the two sexes
For example in humans Breast development is normally limited to females Beard growth is normally limited to males 4-55 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
66
Another example: Feather plumage in chicken
Refer to Figure 4.16 Caused by an autosomal gene Hen-feathering is controlled by a dominant allele expressed in both sexes Cock-feathering is controlled by a recessive allele only expressed in males Genotype Phenotype in Females in Males hh hen-feathered cock-feathered Hh HH 4-56 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
67
Fig. 4.16
68
Sex-limited Traits The pattern of hen-feathering depends on the production of sex hormones If the single ovary is surgically removed from a newly hatched hh female She will develop cock-feathering and look indistinguishable from a male 4-57 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
69
4.2 GENE INTERACTIONS Gene interactions occur when two or more different genes influence the outcome of a single trait Indeed, morphological traits such as height weight and pigmentation are affected by many different genes in combination with environmental factors 4-58 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
70
If these two genes govern two different traits
We will next examine three different cases, all involving two genes that exist in two alleles The three crosses we will perform can be illustrated in this general pattern AaBb X AaBb Where A is dominant to a and B is dominant to b If these two genes govern two different traits A 9:3:3:1 ratio is predicted among the offspring However, the two genes in this section do affect the same trait The 9:3:3:1 ratio may be affected 4-59 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
71
A Cross Involving a Two-Gene Interaction Can Still Produce a 9:3:3:1 ratio
Inheritance of comb morphology in chicken First example of gene interaction Discovered by William Bateson and Reginald Punnett in 1906 Comb types come in four different morphologies Refer to Figure 4.17a 4-60 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
72
4-61 Figure 4.17b The crosses of Bateson and Punnett
Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
73
Thus, the F2 generation consisted of chickens with four types of combs
9 walnut : 3 rose : 3 pea : 1 single Bateson and Punnett reasoned that comb morphology is determined by two different genes R (rose comb) is dominant to r P (pea comb) is dominant to p R and P combination: walnut comb rrpp produces single comb Note: Mendel’s laws of segregation and independent assortment still hold! 4-62 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
74
A Cross Involving a Two-Gene Interaction Can Produce a 9:7 ratio
Inheritance of flower color in the sweet pea Also discovered by Bateson and Punnett Lathyrus odoratus normally has purple flowers Bateson and Punnett obtained several true-breeding varieties with white flowers They carried out the following cross P: True-breeding purple X true-breeding white F1: Purple flowered plants F2: Purple- and white-flowered in a 3:1 ratio These results were not surprising 4-63 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
75
But these results were 4-64 Figure 4.18
Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
76
Flower color is determined by two different genes:
Thus, the F2 generation contained purple and white flowers in a ratio of 9 purple : 7 white Flower color is determined by two different genes: C (one purple-color-producing) allele is dominant to c (white) P (another purple-color-producing) allele is dominant to p (white) cc or pp masks P or C alleles, producing white color Thus, a plant that is homozygous for either recessive white allele, would develop a white flower 4-65 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
77
The term epistasis describes the situation in which a gene can mask the phenotypic effects of another gene Epistatic interactions often arise because two (or more) different proteins participate in a common cellular function Enzyme C Enzyme P Colorless precursor Colorless intermediate Purple pigment The recessive c allele encodes an inactive enzyme The recessive p allele encodes an inactive enzyme If an individual is homozygous for either recessive allele It will not make any functional enzyme C or enzyme P Therefore, the flowers remain white 4-66 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
78
An example of epistasis- 9:3:4
BC bC Bc bc 1⁄4 BBCc BbCc BBcc Bbcc bbcc bbCc BbCC bbCC BBCC 9⁄16 3⁄16 4⁄16 Sperm Eggs c/c “masks” B/- therefore C is epistatic to B
79
A Cross Involving a Two-Gene Interaction Can Produce an 8:4:3:1 ratio
Inheritance of the Cream-Eye allele in Drosophila Discovered by Calvin Bridges He identified a rare fly with cream-colored eyes, in a true-breeding culture of flies with eosin eyes This could be explained in one of two ways 1. A new mutation changed the eosin allele into a cream allele 2. A mutation occurred in another gene that modified the expression of the eosin allele 4-67 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
80
Testing the Hypothesis
Cream-colored eyes in fruit flies are due to the effect of a second gene that modifies the expression of the eosin allele Testing the Hypothesis Refer to Figure 4.19 4-68 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
81
Figure 4.19 4-69 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
82
The Data Cross Outcome 4-70 P cross: Cream-eyed male X
wild-type female F1: all red eyes F1 cross: F1 brother X F1 sister F2: 104 females with red eyes 47 males with red eyes 44 males with eosin eyes 14 males with cream eyes 4-70 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
83
Interpreting the Data Cross Outcome 4-71 P cross: Cream-eyed male X
wild-type female F1: all red eyes F1 cross: F1 brother X F1 sister F2: 104 females with red eyes 47 males with red eyes 44 males with eosin eyes 14 males with cream eyes F2 generation contains males with eosin eyes This indicates that the cream allele is not in the same gene as the eosin allele 4-71 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
84
Interpreting the Data One possibility is that the cream allele is an autosomal recessive allele C = Normal allele Does not modify the eosin phenotype ca = Cream allele Modifies the eosin color to cream Refer to Figure 4.19 4-72 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
85
Figure 4.19 4-73 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
86
4-74 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
87
The specific modifier allele, ca, can modify the phenotype of the eosin- but not the red-eye allele
The eosin can be modified only when the ca allele is homozygous The predicted 8:4:3:1 ratio agrees reasonably well with the data of Bridges 4-75 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display
Similar presentations
© 2025 SlidePlayer.com. Inc.
All rights reserved.