1. Presenter Anna Daily Ph.D.-Senior Scientist March 9, 2012 Breast Health Providers Conference

2. Why A New Approach to Breast Cancer Screening?  Of women age 40 and over with insurance coverage only 50% participate in yearly screenings.  Why women don’t participate:  Dense Breast Tissue  Lack of Facility Access  Some find procedure painful  Cost prohibitive for uninsured  False Positive rate of 5% per exam= 50% over per patient lifetime

3. Ascendant Diagnostics is Developing: An inexpensive, highly accurate test that can diagnose cancer at earlier stages is highly sought after  A Low Cost  Non-Invasive  Screening Method for Breast Cancer  Detects proteins present in tear samples  Based on technology licensed from UAMS

4. Why Tears?  Easy and Quick to Collect  Pain Free  Non-invasive  Contain Protein in High Concentrations  Easy to Prep for Test Analyze Tears by Mass Spectrometry Collect Tear Sample Look for Differences

5. Mass Spectrometry Private Industry  Quest  LabCorp  PeaceHealth Labs Drug Testing Monitoring Pain Medication Monitor metabolic disorders Separates samples based on Size Increasing size Intensity Bigger peak=more protein

6. Genomics vs. Proteomics DNA Sequences Protein Structure and Function Predictive of Risk Not Diagnostic Predictive of Risk Not Diagnostic Diagnostic

7. Imaging is the Gold Standard Tear Testing 80% Sensitive 75% Specific 90% Sensitive 90% Specific $75.00 per test vs. $200 for mammogram

8. Regulatory Pathway CLIA: Clinical Laboratory Improvements Amendment

9. Timeline 2011 2012 2013 2014 2015 Licensed Technology; Business Case; Established Strategic Partnerships Protein Biomarker Identification; 2 cohorts of 300 patients; establish more partnerships; develop CLIA protocol Clinical Validation; Marketing and Sales Team Build up Contract Development of POC Lab on a Chip and Contract Development of Reagents; Sales and Mktg. Other Cancer biomarkers; lines between treatment and level of protein

10. Collaborations The Breast Center – established February 2012 – 300 tear samples over the next 2 years Winthrop P. Rockefeller Cancer Institute Women’s clinic (UAMS) – In Progress – 300 tear samples over the next 2 years Peace Health Labs – Established August 2011 – Aid and in development and testing of CLIA lab protocol University of Arkansas Statewide Mass Spectrometry Facility – established February 2011 – Acess to mass spectrometry instrumentation – Collabroations on Grant applications – Aid in development of CLIA based protocol

11. Management Team

12. Scientific Advisory Board

13. Contact Information  Omid Moghadam CEO 479-571-2592 omid.moghadam@ascendant- diagnostics.com omid.moghadam@ascendant- diagnostics.com  Anna Daily Ph.D. Scientist 479-571-2592 anna.daily@ascendant-diagnostics.com