1. Mendelian Inheritance & Principles November 20, 2007 BIO 184 Dr. Tom Peavy (Chapter 2- Brooker Text)

2. –Theory of Pangenesis –Theory of Preformationism –Blending Theory of Inheritance Central Question How are traits inherited?

3. Theory of preformationism (homunculus)

4. Human Genome Project Skip Ahead to the 21 st Century: We know the genetic information (DNA) is organized within Chromosomes We receive one homologous chromosome from each parent ♂n + ♀n = 2n (new unique individual) http://www.ncbi.nlm.nih.gov/

5. P CrossF 1 generationF 2 generationRatio Tall X dwarf stem All tall787 tall, 277 dwarf 2.84:1 Round X wrinkled seeds All round5,474 round, 1,850 wrinkled 2.96:1 Yellow X Green seeds All yellow6,022 yellow, 2,001 green 3.01:1 Purple X white flowers All purple705 purple, 224 white 3.15:1 Axial X terminal flowers All axial651 axial, 207 terminal 3.14:1 Smooth X constricted pods All smooth882 smooth, 229 constricted 2.95:1 Green X yellow pods All green428 green, 152 yellow 2.82:1 DATA FROM MENDEL’S MONOHYBRID CROSSES

6. Mendel’s Law of Segregation During gamete formation, the paired factors segregate randomly so that half of the gametes received one factor and half of the gametes received the other

7. Dihybrid Crosses Crossing individual plants that differ in two traits For example –Trait 1 = Seed texture (round vs. wrinkled) –Trait 2 = Seed color (yellow vs. green) There are two possible patterns of inheritance for these traits

9. P CrossF 1 generationF 2 generation Round, Yellow seeds X wrinkled, green seeds All round, yellow 315 round, yellow seeds 101 wrinkled, yellow seeds 108 round, green seeds 32 green, wrinkled seeds DATA FROM DIHYBRID CROSSES

10. Interpreting the Data The F 2 generation contains seeds with novel combinations (ie: not found in the parentals) –Round and Green –Wrinkled and Yellow These are called nonparentals Their occurrence contradicts the linkage model

11. P CrossF 1 generationF 2 generationRatio Round, Yellow seeds X wrinkled, green seeds All round, yellow315 round, yellow seeds 101 wrinkled, yellow seeds 108 round, green seeds 32 green, wrinkled seeds 9.8 3.2 3.4 1.0 DATA FROM DIHYBRID CROSSES

12. Law of Independent assortment During gamete formation, the segregation of any pair of hereditary determinants is independent of the segregation of other pairs

13. Problem Solving

14. TtYy x TtYy Dihybrid Cross

16. TtYy x ttyy What is the expected phenotypic ratio among offspring? Problem Solving

17. Tt RR x Tt RR What fraction of the progeny will be Tall and Round?

18. (pg 38)

19. Alternative = Forked Line Method

22. Tt RR Yy x Tt RR Yy What fraction of the progeny will be Tall, Round, and Green?