1. Introducing the EASY-nLC™
Split-less, nano-flow HPLC system, for proteomics from

2. Copyright © Proxeon Biosystems A/S
Overview
The EASY-nLC™ is:
Complete
Configured
Compact
EASY to
install
learn
operate
Performance
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Complete
1 cooled autosampler
96 or 384 well plates
Single or double height
Wash & Waste bottles
switching valves
nano-flow sensor
nano-litre pumps
Built-in PC & software
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4. EASY2-nLC™ (with flow control) 2-column
AFC™
Advanced Flow Control
system
Solvent A
Solvent B
Waste
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5. EASY2-nLC™ (with flow control) 1-column
AFC™
Advanced Flow Control
system
Solvent A
Solvent B
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6. EASY2-nLC™ (with flow control)
2-column
1-column
Waste
Waste
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Compact
Small footprint
35cm wide (14”)
38cm deep (15”)
45cm high (18”)
Touch-screen controlled
built-in computer
no keyboard or mouse required
Light
35 kg, all inclusive
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Easy to install
Only a few cables required
contact closure
[network is optional]
Power
Software is
integrated,
pre-installed and
up-to-date
Solvent purging, air removal and column flushing is fully automated
Less than 4 hours from power up to first analytical data
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Easy to learn
Logical software structure
Practice oriented work flow
Results oriented parameters
E.g. volume & flow rates, not hardware control
Intuitive graphical user interface
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Easy to operate (1)
What You See Is What You Get
Simple design
Logical layout
Clear separation in compartments
Modular components
Easy access to all parts of instrument
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Easy to operate (2)
Automated maintenance scripts
Intelligent trouble shooting software
Web interface for software upgrades
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Easy to operate (3)
Split-less, nano-flow means:
Less solvent use
Less waste
Robust design & Intelligent software control means:
Less support
Less maintenance
More uptime M T W F S 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 M T W F S 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30
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Example (1)
Mass spectrometer
Finnigan LCQ Classic
Pre-column
2cm, 75µm ID, Reprosil-Pur C18-AQ. 5µm
Analytical column
10cm, 75µm ID, Reprosil-Pur C18-AQ. 3µm
Solvents
A: 0.005% HFBA, 0.4% acetic acid, water
B: 0.005% HFBA, 0.4% acetic acid, 90% acetonitril, water
Amount injected
100 fmol tryptic digested BSA
HPLC gradient
300 nl/min, %B in 25 minutes
6 out of 36 consecutive runs .
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Example (2)
Carry-over
< 0.003%
Sample pickup
From 0.2µl to full loop in 0.01µl steps
Loop size adjustable
RSD < 0.5%
Peak widths (FWHM)
< 10-15s at 50%
Reproducibility
RT RSD < 0.7%
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Summary
The EASY-nLC™ is:
A fully integrated and complete instrument
With true nano-flow and on-line monitoring
Designed from the ground up to be easy and intuitive to operate
A nano-LC system for the 21st century
Copyright © Proxeon Biosystems A/S