1. Bacteria (and other small topics) The Good, The Bad, and The Ugly

2. Arrangement Groups Chains: strepto- Pairs: diplo- Irregular clusters:staphylo- Foursomes: tetrad Cubes: sarcina

3. Shape Groups Spheres – coccus (cocci) Rods – bacillus (bacilli) Spirals, bent rods, and corkscrews – spirillum (spirillae)

4. Gram Stain Reaction Pink/red – Gram negative Blue/purple – Gram positive Mixture – Gram variable See Gram Stain Procedure on next slide

5. Fix the bacteria. Bathe with Crystal Violet for 1 minute. Rinse with water. Bathe with Gram’s Iodine for 1 minute. Rinse with water. Rinse with drops of ethanol until runoff is clear. Bathe with Safranin for 1 minute. Rinse with water. Flame dry. View, sketch and describe.

6. 1. What is this?

7. Gram negative diplococci

8. 2. And this?

9. Gram positive bacillus

10. 3. And these are…..

11. Gram negative spirillae

12. 4. How about these?

13. Gram positive streptobacilli

14. 5. These are……..

15. Gram positive spirillae

16. 6. What are these? They’re actually Gram positive staphylococci

17. 7. What would you call these?

18. Gram positive streptococci

19. 8. And finally, these are…..

20. Gram positive sarcina (cocci)

21. So how did you do? 5 out of 8 would be acceptable.