1. Lab 9. Overview  Instructor checks pre-labs. in lab notebook  Discussion of exam, sickle-cell disease write up, & the independent projects.  Basic Bacteria Overview.  Examine 5 prepared slides & 3 culture plates  Isolation streaking exercise  Antibiotics exercise with bacterial lawns  Create a bacterial model & examine model  Examine and sketch your plant cultures.  Discuss ind. proj. w/ instructor before leaving. Read book & make eukaryotic model by next week.

2. Bacterial Cell

3. Cell Walls

4. Cell Shape & Arrangement

5. Bacterial Colonies & Streaking Streaking dilutes the bacteria so that single cells can grow into isolated colonies.

6. Reminders for the Lab  READ the instructions (  ) in the manual  The tops on broth tubes are NOT leak proof.  Ceramic sterilizers are HOT.  Remember to sterize your loop between each streak.  Put samples on the medium, not on the lid.  Be careful with the Bunsen burners and open flames. Do not set yourself on fire.  Do not roast the spreaders in the flame.  Do not put a flaming or hot spreader into the alcohol beaker.