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Parallel and Simultaneous Dispensing of Nanoliter Volumes for the Production of BioChips and Diagnostic Tests Bellows Dispensing Technology
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Bottom valves are opened Dispenser is ready for dispensing Top valves are closed Bellows are filled and expanded Connections to individual reagent reservoirs Bottom valves Top valves Bellows Dispenser needles
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bottom valves opened Lilliput Plate is positioned top valves are closed Bellows are filled and expanded Connections to individual reagent reservoirs
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bottom valves opened Dispensing starts top valves are closed mechanical compression of the bellows Connections to individual reagent reservoirs
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bottom valves opened trackdown of the Lilliput chip Connections to individual reagent reservoirs Dispensing ready top valves are closed mechanical compression of the bellows
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bottom valves opened Removal of the Lilliput chip top valves are closed mechanical compression of the bellows removal of the Lilliput chip Connections to individual reagent reservoirs
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Valve operation top valves are closed Connections to individual reagent reservoirs bottom valves are closing
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Valve operation top valves are closed Connections to individual reagent reservoirs
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bottom valves are closing Valve operation top valves are closed Connections to individual reagent reservoirs
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bottom valves are closing Valve operation top valves are closed Connections to individual reagent reservoirs
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bottom valves closed Valve operation top valves are opening Connections to individual reagent reservoirs
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bottom valves closed Valve operation top valves are opening Connections to individual reagent reservoirs
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bottom valves closed Valve operation top valves are opening Connections to individual reagent reservoirs
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bottom valves closed Bellows ready for refill top valves are open Connections to individual reagent reservoirs
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bottom valves closed Bellows ready for refill top valves opened Connections to individual reagent reservoirs
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bottom valves closed Bellows refill top valves opened Connections to individual reagent reservoirs Bellows expand
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bottom valves closed Bellows refill top valves opened Connections to individual reagent reservoirs Bellows expand
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bottom valves closed Bellows refill top valves opened Connections to individual reagent reservoirs Bellows expand
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bottom valves closed Bellows refill top valves opened Connections to individual reagent reservoirs Bellows expand
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bottom valves closed Bellows refill top valves opened Connections to individual reagent reservoirs Bellows expand
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bottom valves closed Bellows refill top valves opened Connections to individual reagent reservoirs Bellows expand
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bottom valves closed Bellows refill top valves opened Connections to individual reagent reservoirs Bellows expand
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top valves are closing bottom valves are opening Valve operation Connections to individual reagent reservoirs
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Valve operation Connections to individual reagent reservoirs top valves are closing bottom valves are opening
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bottom valves opened Dispenser ready for operation top valves closed Connections to individual reagent reservoirs
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Specifications Accuracy:VC < 2% Volumes:200nl up to 2000nl [2µl – 100µl] Bellows size:3µl up to 2ml Channels:1 to 384 in parallel Reagents:1 to 192 different solutions simultaneously Operation Modes:- constant dispensing from reservoirs - aspirate and dispense Outlet:- stainless steel needles (teflon or paralene coated) - pipette tips Movement Control:Stepper motor. Resolution 14,9 steps to squeeze 1 nl References:- manufacturing of 384 well plates - manufacturing of Lilliput Chip - manufacturing of 96 well Micro Titer Plates
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Results of 30 doses per each channel: 123456789101112 A 497496490518504 515494500495515499ave. / nl 3.72.31.22.13.71.42.31.01.1 2.52.4STD / nl B 491489511497490489496503493517497506ave. / nl 1.81.11.73.30.91.60.81.31.01.31.21.4STD / nl C 497510495502498497501504501505514509ave. / nl 2.70.92.65.02.32.72.42.20.93.63.41.5STD / nl D 486493502491511497 504516501519512ave. / nl 4.80.73.40.93.11.82.60.92.21.01.61.3STD / nl E 504485492511482517492508501505519508ave. / nl 5.34.70.71.73.41.11.70.92.70.72.82.4STD / nl F 495509519514485496489493503502505487ave. / nl 0.81.14.11.51.22.51.52.32.95.01.41.3STD / nl G 493488482493489478495484494497498493ave. / nl 3.05.41.91.11.32.21.31.04.10.81.04.0STD / nl H 473476474475480472496476478474484481ave. / nl 1.91.81.01.61.54.71.21.41.61.73.31.6STD / nl Validation Data
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