1. Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings. Brock Biology of Microorganisms, Twelfth Edition – Madigan / Martinko / Dunlap / Clark Genetic Engineering Chapter 11 Questions by Dr. Michelle Furlong and Dr. Francine Norflus

2. Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings. Chapter 11: Genetic Engineering Question: A typical restriction enzyme will recognize a sequence of DNA that contains how many bases? A. 4-8 B. 10-14 C. 18-20 D. more than 25

3. Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings. Question: Restriction enzymes can cleave the DNA producing _______________. A. single-stranded overhangs B. blunt ends C. both single-stranded overhangs and blunt ends D. neither single-stranded overhangs nor blunt ends Chapter 11: Genetic Engineering

4. Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings. Chapter 11: Genetic Engineering Question: Ethidium bromide is used in _______________ to be able to _______________. A. Southern Blots; verify the gene is present B. Cloning; determine if DNA was taken up by cells C. Electrophoresis; stain and see the DNA

5. Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings. Chapter 11: Genetic Engineering Question: In cloning, the vector and foreign DNA can be ligated together if _______________. A.both were cut with the same staggered cutting restriction enzyme B.the vector was cut with a staggered cutting restriction enzyme and the foreign DNA cut with a blunt cutting restriction enzyme C.both were cut with a blunt cutting restriction enzyme D.a and c

6. Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings. Chapter 11: Genetic Engineering Question: Which of the following terms do not relate to sequencing DNA when you start with a DNA template? A. radioactive primers B. deoxyribonucleotide triphosphates C. dideoxyribonucleotide triphosphates D. DNA polymerase E. probe

7. Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings. Chapter 11: Genetic Engineering Question: Dideoxynucleotides are different from normal nucleotides because the dideoxynucleotides _______________. A. do not contain a 3’ hydroxyl group B. do not contain a 2’ hydroxyl group C. contain both a 2’ and 3’ hydroxyl group D. contain a 5’ hydroxyl group

8. Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings. Chapter 11: Genetic Engineering Question: After setting up the DNA sequencing reaction, which of the following would not be used or performed to determine the sequence of the template DNA? A. electrophoresis B. ethidium bromide C. radioactivity D. X-ray film E. fluorescence

9. Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings. Chapter 11: Genetic Engineering Question: In sequencing you need _______________, whereas in PCR you need _______________. A. 2 primers; 2 probes B. 2 probes; 2 primers C. 2 primers; 1 primer D. 1 primer; 2 primers

10. Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings. Chapter 11: Genetic Engineering Question: The uptake of DNA into bacteria is called _______________. A.transformation B.transfection C.transduction D.conjugation

11. Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings. Chapter 11: Genetic Engineering Question: An antibiotic resistance gene in a plasmid is used in cloning to find the cells that contain _______________. A. the plasmid B. the foreign gene that you are trying to clone C. the recombinant plasmid

12. Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings. Chapter 11: Genetic Engineering Question: When labeling DNA with a radioactive probe, _______________. A.both 32 P and 35 S can be used because DNA contains both phosphate and sulfur B.only 32 P can be used because DNA does not contain sulfur C.only 35 S can be used because DNA does not contain phosphate

13. Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings. Chapter 11: Genetic Engineering Question: Which of the following is NOT an essential feature for a vector that will be used in cloning to have? A. origin of replication B. restriction enzyme sites C. a gene for selection such as an antibiotic resistance gene D. the LacZ gene