1. Evaluation of Cloned DNA for B19 Genotypes Dr Jacqueline Fryer, Division of Virology NIBSC

2. Genetic Diversity of Parvovirus B19: Analysis of the NS1/VP1 Region A6 Servant et al., J. Virol. 2002

3. Plasmid Information PlasmidGenotype Insert size (bp)VectorSource pB19-N815224 pCR-Blunt II-TOPO (Kan) Zhi et al. 2004 pA6-cl824915 pCR-Blunt II-TOPO (Kan) Nguyen et al. 2002 pV935028pcDNA2.1 (Amp) Nguyen et al. 1999 pD91.135035pcDNA3.1 (Amp) Servant et al. 2002

4. Evaluation of Plasmids Transformed each plasmid & maxi preps grown up for each clone Transformed each plasmid & maxi preps grown up for each clone DNA sequence analysis of insert/vector region determined DNA sequence analysis of insert/vector region determined Restriction enzyme digestions of clones Restriction enzyme digestions of clones Analysis by consensus real-time PCR assay Analysis by consensus real-time PCR assay

5. Analysis of Plasmid DNAs by Consensus Assay for Parvovirus B19

6. Distribution and Use of Clones to OMCL Network and Manufacturers To be distributed through the EDQM PTS for parvovirus B19 DNA To be distributed through the EDQM PTS for parvovirus B19 DNA To be used for the validation of assays & material not to be distributed beyond the recipients laboratory To be used for the validation of assays & material not to be distributed beyond the recipients laboratory MTA with NIH for pB19-N8, pA6 MTA with NIH for pB19-N8, pA6 Hôpital Armand Trousseau permission to supply pV9 & pD91.1 Hôpital Armand Trousseau permission to supply pV9 & pD91.1