1. Restriction Enzymes DNA is a long chain of nucleotides. AluI recognises AGCT, and fixes itself on its target sequence. It cuts after the G to give Blunt Ends AGCT TCGA

2. Restriction Enzymes DNA is a long chain of nucleotides. AluI recognises AGCT, and fixes itself on its target sequence. It cuts after the G to give Blunt Ends AG CT TC GA AGCT TCGA

3. Restriction Enzymes EcoRI recognises GAATTC, and fixes itself on its target sequence. It cuts after the G to give Sticky Ends GAATTC CTTAAG

4. Restriction Enzymes EcoRI recognises GAATTC, and fixes itself on its target sequence. It cuts after the G to give Sticky Ends GAATTC CTTAAG G AATTC CTTAA G