1. Canine Adenovirus - 2 Case C08-0353 Julia Conway Resident Veterinary Anatomic Pathology Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

2. Case history   8-week old female yorkie from Miami pet store   Shipped from South America   1/5 BCS   Unknown vaccination status   Died with clinical signs of respiratory infection   Sample submitted – –Lung www.wikipedia.com Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

3. Morphologic Diagnosis  N ecrotizing bronchiolitis  Fibrinosuppurative interstitial pneumonia Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

12. Canine Adenovirus  CAV-1 –Infectious canine hepatitis –Can cause pulmonary pathology  CAV-2 –Primary adenoviral pneumonia rare –Associated with immunosuppression –Often in conjunction with co-infection (CDV & CPI) or secondary infection Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

13. Canine Adenovirus 2  Genus - Mastadenovirus  Family - Adenoviridae  icosahedral  double stranded  DNA virus  Antigenically related to CAV-1 Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

14. CAV-2  Clinical and gross findings –inapparent to nonspecific respiratory – bronchopneumonia and interstitial pneumonia  Epitheliotropic – bronchiolar epithelium  Bronchial epithelium  pneumocytes  Transmitted –Oral, nasal and ocular secretions Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

15. PCR Results  CAV-2 positive  Canine Distemper Virus positive  Canine parainfluenza positive  CAV-1 negative Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

17. Morphologic Diagnosis  N ecrotizing bronchiolitis, subacute, multifocal, moderate to severe, with large, basophilic, intranuclear inclusions (etiology consistent with Adenovirus) and rare eosinophilic intranuclear and intracytoplasmic inclusions (etiology consistent with Morbillivirus), lung.  Interstitial pneumonia, fibrinous and suppurative, subacute, diffuse, moderate with large, basophilic, intranuclear inclusions consistent with adenovirus, lung. Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

18. Acknowledgements:  Jeff Abbott, DVM, PhD, Dipl. ACVP  William Castleman, DVM, PhD, Dipl. ACVP  Katie Kingham, UF VMC Histology staff  EM – Lou Ann Miller, Center for Microscopic imaging – UIUC  Fellow Residents Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

19. QUESTIONS?