1. Who’s Who in the Microbe World?

2. Remember the Six Key Concepts of Marine Microbes Microbes are everywhere, they are extremely abundant and diverse Most marine microbes are beneficial. Life on earth could not exist without microbes. Microbes significantly impact our global climate. New discoveries everyday in the field of microbial oceanography Marine microbes are very small and have been around for a long time.

3. http://www.lbl.gov/Science- Articles/Archive/JGI-microbe-clues.html 1 http://www.sciencefriday.com/program/arch ives/200806132

4. cyanobacteria

5. photosynthetic

6. Estimated that in one out of five breaths the oxygen is coming from this

7. Abundant number in trillion trillions

8. Discovered about 20 years ago It’s 20 th year discovery was celebrated with a two day event!

9. Click HERE to HEAR ABOUT THE DISOVERY….. Runs about 5 minutes http://www.npr.org/templates/player/m ediaPlayer.html?action=1&t=1&islist=f alse&id=91448837&m=91461513 Sallie (Penny) Chisholm Professor of Civil and Environmental Engineering and Biology; Lee and Geraldine Martin Professor of Environmental Studies Director, MIT Earth System Initiative http://mitworld.mit.edu/video/421/ http://www.scq.ubc.ca/flow-cytometry-a- technology-to-count-and-sort-cells/

10. You’ve just met Prochlorococcus

11. 2 http://www.nature.com/nrmicro/journal/v5/n 10/images/nrmicro1752-f4.jpg

12. Has about 1,300 genes (as compared to our 30,000) Genome contains no viral DNA and no junk DNA

13. Archae

14. Feeds off dead organic matter in ocean (heterotrophic)

15. Estimated to be 25% of life in the ocean (by number of organisms)

16. Environmental Sample Processor (ESP) data Key words: ESP and microbes

17. You’ve just met Peligabacter

18. 3 http://www.cfsan.fda.gov/~frf/pyro.gif

19. Algae dinoflagellate

20. Chemical reaction between luciferan and luciferase

21. http://www.elyunque.com/adven/biolady.jpg

22. You’ve just met Pyrodinium bahamense

23. 4

24. photosynthetic

25. Symbiotic relationship with coral Coral bleaching Key words: symbiotic with coral

26. You’ve just met zooanthellae

27. 5

28. Fish and zooplankton can graze on it

29. Too small to see with a school microscope

30. Sediments responsible for the white cliffs of Dover http://farm1.static.flickr.com/119/290719612_5a27cbaf61.jpg

31. Sometimes visible from space in as an algal bloom http://earthobservatory.nasa.gov/Study/Coc coliths/bering_sea.html Key words: ocean color blooms

32. You’ve just met Coccolithophore

33. 6

34. cyanobacteria

35. Fixes atmospheric nitrogen

36. Sometimes called sea sawdust

37. Brown algal bloom can look like foamy pollution. Keyword: sea sawdust

38. You’ve just met Trichodesmium

39. 7

40. diatom

41. Some species produce toxin domoic acid

42. California blooms responsible for sea lion deaths in 1999 and recurrent shell-fish contamination.

43. Can cause Amnesic Shellfish poisoning in humans

44. ESP data for related species Key words: esp and mbari check out the 2007 harmful algal data

45. You’ve just met Pseudonitzschia australis

46. 8

47. Eukaryote dinoflagellate

48. Can cause Harmful Algal Bloom (HAB)

49. It fluoreses green if enzyme alkaline phosphatase is present Its chloroplasts fluorese red.

50. ESP DATA GO to http://www.mbari.org/ESP /field_data/2007/HAB_20 07.htm

51. You’ve just met Alexandrium tamarense

52. ASSESSMENT classification (flips to information) Each group of students will choose a different microbe, research and construct a poster flip-board with the following information:  picture or drawing of organism  classification (algae {diatom, dinoflagellate, etc}, bacteria)  role in environment  how are they identified in environment  miscellaneous information  More “flips” may be included PICTURE OF ORGANISM (flips to key word) Role in environment (flips to information) How are they identified (ESP, remote sensing, other method) (flips to information) Miscellaneous information (flips to information) The answer should be taped on a piece of 8.5X 11 inch paper to the back of the poster (for display later)

53. Having trouble picking a microbe?  Help clean up industrial and environmental problems (oil spills, heavy metals)  Microbes are used in PCR  Are found in extreme environments (hydrothermal vents)  Can be bioluminescent  Are found in our digestive tracts (and the tracts of animals too)  Are important in the production of bread, cheese, yogurt, beer and wine)  Are found deep in the ocean around whale falls  Cause disease  Cause harmful and not so harmful algal blooms  NASA is searching for microbes on other planets

54. The following two slides have information that may need to be printed out for those classes without internet access

55. ESP bacteria data that would need to be provided if students didn’t have access to internet

56. ESP algae data that would need to be provided if students didn’t have access to internet control Alexandrium tamarense/catenella Pseudo-nitzschia multiseries P. multiseries/ pseudodelicatissima P. australis Heterosigma akashiwo