1. Filling rack matrix with solvent 18.05.2015 11:28 Needs version 1.1.3.8A

2. Filling rack matrix with solvent 18.05.2015 11:28 1.„Enable SampleTrack“ is unchecked„Enable SampleTrack“ is unchecked 2.„Manually destination selection“ is checked„Manually destination selection“ is checked 3.„Clean... “ is unchecked„Clean... “ is unchecked Step 1: Check Settings

3. Filling rack matrix with solvent 18.05.2015 11:28 Open procedure editor dialog Two main procedures will be defined: 1. Fill syringe with the total solvent amount 1200µl for 12 tubes. Name: „Load Syringe DMSO 1200.gsp“ 2. Dispense partial solvent amount 100µl into one destination. Name: „Dispense Volume 100.gsp “ (with sub procedure „Sub Disp Volume 100.gsp “)

4. Filling rack matrix with solvent 18.05.2015 11:28 2. Select Source Type: „Solvent in Tray“. 3. Select your own values for: - Solvent name - Volume (Speed...) Hint: Never forget „Update“ and „Save“ after modifications. 1. Check the file name „Dispense Volume 100.gsp “ Step 2: Edit procedure „Load Syringe DMSO 1200.gsp“

5. Filling rack matrix with solvent 18.05.2015 11:28 Step 3a: Edit dispense sub procedure „Sub Disp Volume 100.gsp “ Press the button „Edit“ to edit or create a procedure of the type „Dispense“.

6. Filling rack matrix with solvent 18.05.2015 11:28 Select your own values for: - Volume - Z offset height (Speed...). Check the file name „Sub Disp Volume 100.gsp“. Step 3a: Edit dispense sub procedure „Sub Disp Volume 100.gsp “

7. Filling rack matrix with solvent 18.05.2015 11:28 Select the Source Type: „Gas Gap“ Select your Dispense procedure. Check the file name „Dispense Volume 100.gsp “ Volume = 0 Step 3b: Edit dispense main procedure „Dispense Volume 100.gsp “

8. Filling rack matrix with solvent 18.05.2015 11:28 Start Automation. Step 4: Start Automation to fill sample loop 1.Select procedure „Load Syringe DMSO 1200.gsp“. 2. Select one dummy source and destination position (not used inside procedure!). Hint: Solvent not used in procedure! 3. Create Order and continue with „OK“.

9. Filling rack matrix with solvent 18.05.2015 11:28 Do NOT start automation! Syringe would be homed zero. Open Dialog „Create new Orders“. Step 5: Create Orders to fill rack matrix. Procedure finished OK: solvent filled in Sample loop.

10. Filling rack matrix with solvent 18.05.2015 11:28 Step 6: Create Orders to fill rack matrix 1.Select procedure „Dispense Volume 100.gsp“. 2. Select 12 source and destination positions (destinations will be used). Hint: Solvent not used in procedure! 3. Create Orders and close dialog with „OK“.

11. Filling rack matrix with solvent 18.05.2015 11:28 Do NOT start automation! Syringe would be homed to zero. Use menu „Service Tools“. Sub menu: „Prepare all Orders“. Step 7: Start preparation to fill rack matrix

12. Filling rack matrix with solvent 18.05.2015 11:28 Step 7: Running preparation