1. Spit Lab

2. Carbohydrate StarchPolysaccharide Monosaccharide Monomer Macromolecule Glucose

3. Indicators Iodine tests for the presence of starch Light brown = no starch Dark purple or black = starch

4. Indicators Glucose test strips test for the presence of glucose (Insert the specific color scale here)

5. How to make a spit tube: Demonstration Keep cracker in your mouth until it is runny No chunks Very wet with spit You will want to swallow it, but don’t!

6. Part 1: Test for starch

7. Part 2: Test for sugar

8. Part 3: Effect of temperature

9. NegativeSmall amount of glucose Medium amount of glucose High amounts of glucose

10. Enzyme Activity Temperature degrees Fahrenheit 050100150 200 250 300 100 75 50 25 0

11. Glucose concentration/ Enzyme activity (Add units) Temperature degrees Fahrenheit 050100150 200 250 300