1. BGYB30 Mammalian Physiology Today: –Course Organization –Intro to Cellular Physiology Next Lecture: –Intro to Nervous system organization and the neuron

2. BGYB30 Mammalian Physiology Nerve Muscle Endocrine

3. Course Organization Bryan Stewart, Ph.D. Lectures –Tuesday & Thursday 12 noon (Lec 01) 1 pm (Lec 02) My Office Hours –Tuesday 2-3 pm; Wed 11am-12 noon, S540C –By appointment –Tutorial time slots

4. Teaching Assistant 1.Afshan Ghesmy-Bahkt –Office hours: TBA

5. Course Organization Tutorials –every third Thursday 5-7 pm alternating with BGYB10 and BGYB50 BGYB30 Tutorials –September 9 - cancelled –September 30 –October 21 –November 11 Question & Answers Extended Office Hours

6. Course Organization Email: bgyb30@utsc.utoronto.ca –I cannot answer course related email sent to my regular email address Communications

7. Course Organization The Intranet Webpage –Syllabus –Announcements –Lecture notes Communications

8. Course Organization I will try to post lectures 24 hrs in advance I reserve the right to add or delete material from the posted notes during the lecture Lecture Notes

9. Textbook –Eckert Animal Physiology 5 th Edition, 2001 by Randall, Bruggren, & French, W.H. Freeman and Company. –All material will be covered in lectures and the text will supplement lectures –Lecture Topics and sections of the textbook will be posted to webpage Course Organization

10. Evaluation: –Mid-term 40% of final mark –Final exam 60% of final mark Multiple choice questions Short Answer questions The final will be comprehensive covering all of the course material Course Organization

11. About me… BSc 1990 –University of Guelph MSc 1991, PhD 1996 –University of Toronto, Dept of Physiology Fellowships: –1996-1998 Stanford University –1998-2001 The Hospital for Sick Children Professor at UTSC since July 2001

12. About me.. Research Interests –Neurophysiology –Synaptic Transmission –Neuromuscular development I use fruit flies as a model system to study the molecular mechanisms used in neural function and development

13. Course Goals: Understand the underlying mechanisms that contribute to nerve, muscle, and endocrine function Understand the cellular basis for higher order physiological systems Appreciate the experimental foundations of physiological knowledge

14. Teaching Philosophy organize material explain (hopefully) why it is interesting Present a model for how to learn the material But it is up to you to learn it!

15. Major Course Topics –Neurophysiology –Muscle Physiology –Endocrinology Why these three? –Together these systems sense and respond to the environment Mamalian Physiology

16. Physiology Physiology – the branch of biology that studies how living things function, especially with respect to the physical and chemical phenomena that are used. THAT MEANS… –A little physics, a little chemistry, a little anatomy…

17. Physiology Asks fundamental questions about how things work –My emphasis is on the cellular level

18. Intro to Cellular Physiology

19. The cellular plasma membrane –Composed of phospholipids & proteins –Selectively permeable barrier Lets essential molecules IN Lets waste products OUT Maintains cell integrity

20. Phospholipid bilayer Gases O 2,CO 2 Ethanol H20H20 Ions :Na +, K +, Ca 2+ Sugars:Glucose Amino Acids ATP Permeability of pure phospholipid

21. Membrane Proteins Mediate Transport Across the Plasma Membrane 1A ATP-powered pump 1B Transporters ATPADP + Pi A Uniporter B Symporter C Antiporter out in

22. Membrane Proteins Mediate Transport Across the Plasma Membrane 2 Ion Channels A Voltage -gated B Ligand -gated closed + - open + + ligand open Membrane voltage

23. 3 G-Protein Coupled Receptors Signal transduction Membrane Proteins Mediate Transport Across the Plasma Membrane

24. 1.ATP-powered pump 2.Transporters 3.Voltage-gated ion channel 4.Ligand-gated ion channel 5.G-protein couple receptor Na + /K + pump Glucose Transporter Na+ channel, K+ channel Neurotransmitter receptor Neurotransmitter & Hormone receptors Transmembrane Protein Example

25. Distinctions 1.Pumps and transporters basal cell function 2.Ion channels and G-protein coupled receptors excitation and inhibition of cell function

26. Question from 2002 final exam Identify three major groups of transmembrane proteins discussed in class, indicate how they function, and give examples of how they act to stimulate and inhibit cellular physiology.

27. Next Lecture –Introduction to Nervous System Organization of the nervous system The neuron