1. What’s Cooking In Microscopy: A Microscopic Look at the Contents of A Refrigerator

2. Darkfield 40x Shark Muscle Cells

3. DAPI Stained Fluorescent Nuclei Within Shark Muscles

4. Adobe Photoshop: Adjust Contrast - Brightness

5. Combined Image Darkfield and Fluorescent Microscopy Done in Layers with Adobe Photoshop

6. Besides their multinucleated status, shark muscle cells have another aspect, clearly visible the polarized and DIC image below. Both 60x images are of the exact same specimen and clearly show the striations present in shark muscle tissue. Obviously, the two techniques produced dramatically different results. DIC shows slight variations in the muscle surface, while the polarized image shows only the most dramatic aspects of the striations in sharp relief. 60x Polarizer: same image! 60x DIC

7. Blue Nuclei - DAPI Red Microtubules - Phallicidin Green N-acetyl glucosamine residues in the cells’ surface - WGA Incidentally, the Shark Tissue was triple-stained with fluorescent dyes (10x)

8. Phase 40 x Recrystalized Salt, 40x: Phony Hoffmann Effect – Light Microscope

9. Focus Through Layers, 100 x Brightfield, oil immersion Red Leaf Lettuce

11. Adobe Photoshop Hue / Saturation Adjust

12. Phase Bottom 2 Images: Angled Stage Setting 3 Video Camera Images of Re-crystallized Sugar, 40x

13. Phase Image Converted to 8 bit grayscale in image J But an Inverted Look- up Table produces dim results! In Photoshop, Contrast and Brightness Adjusted Look-Up Table Applied To Crystallized Sugar

14. This Lookup Table Replaces 176 – 255 Red Values with 0. It Creates a Turquoise Effect where Highlights Occurred in the Image for a colored Look at Sugar’s Crystalline Patterns

15. Onion Cell Nuclei Stained With Iodine - based Fluorescent Stain Random Confocal Slices – Indistinct, Noisy Images… 100 x Magnification

16. To the left: An Averaged Z-projection in Image J compiling all of the confocal slices. It produced a much cleaner image. And with a little doctoring in Photoshop using the diffuse glow filter and the RGB channel mixer, the nuclei and cell membranes become much more distinct

17. Brightfield 40 x Image of Same Onion Skin, a little dull …lots of visual goo in the sample. To the right: A simple convolution applied adds a little interest: 0 1 2 -1 1 1 -2 -1 0

18. But Holy Cow! The convolved onion skin jpeg doctored in Photoshop using Image>Adjust>Curves to give the cell membrane a psychedelic look, crazy man.

19. 1. Image J: Image>Adjust>Brightness / Contrast 2. Image J: Process>Noise>Despeckle 3. PhotoShop: Filter>Smart Blur 4. PhotoShop: Filter>Noise>Despeckle 5. PhotoShop: Touch-up with Black and grey “paint” w/paintbrush 6. PhotoShop: Filter>Noise>Add Noise Fixing Gretchen…

20. The End