1. Female Aedes aegypti taking a blood meal on the arm of Dr. Edwards

2. Eggs (5 days to 1 year) Larva (~10 days) Pupa (1-2 days) Adult (1 week-2 months)

3. Vectors of human disease Infected blood meal Pathogens ingested Pathogens reproduce Next blood meal Pathogens transmitted through saliva Pathogens Ingested Pathogens Injected

4. Mosquitoes enjoying an artificial blood meal

5. Parafilm membrane Cow blood Feed mosquitoes

6. Esophagus : No carboxypeptidae gene expression Carboxypeptidase gene expression in the midgut cells Cow blood

7. Why did we think we had cloned a real; Carboxypeptidase A gene?

8. Fluorescent Anopheles gambiae mosquito larvae

9. Whole-mount in situ hybridization Control

10. Follicular Epithelium ~ 10hr PBM Vitelline envelope 15a-1: Eggshell Gene Vitelline envelope

11. Anti-LaCrosse Protein MxA Human Anti-Viral Gene Induced by IFN-  /  Inhibits virus transcriptase Other antiviral activities?

12. Micro-inject embryos with DNA constructs

13. SYBR green stained gel of mosquito genes expressed after a blood meal. PCR by Priscilla Mc. Dowell ‘01