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Published byGodfrey Baker Modified over 9 years ago
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Tumaini (CRT) Inc. Efficacy of a New Slow Release Mosquito Larvicide containing Novaluron
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SLOW RELEASE MOSQUITO LARVICIDE DETAILS Larvicide contains the active ingredient novaluron and utilizes novel formulation technology which has been patented in numerous countries Formulation controls rate of release of novaluron into water column increases solubility of novaluron in water from 3 ppb to 10-15 ppb Formulation also promotes dispersion of novaluron throughout the water column products will be available in several different sizes and shapes to address different market needs all products have same recipe and ai 1.2g/kg novaluron Tradename for larvicide products are: Mosquiron 0.12P and Mosquiron 0.12CRD
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Mosquiron 0.12P pastilles (2 sizes) Mosquiron 0.12CRD controlled release device MOSQUIRON PRODUCT DETAILS
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Mosquiron 2010 Field Trials PURPOSE: Assess efficacy and duration of Mosquiron products when used under simulated field conditions and in different regions of the US and Canada
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Experimental Design Needed to take into consideration the mode of action of novaluron Novaluron is a chitin synthesis inhibitor and effects can be cumulative; larvae can appear healthy and mortality may not be observed until adults emerge from pupal case At high concentrations of novaluron (>5 ppb) effect on larvae can be rapid – 24 hours Mortality can still occur at pupal eclosion caused by incomplete separation from pupal case – adult drowning Field Trial Locations Canada – Portage la Prairie MB, Abbotsford BC US – Marshall WI, Meansville GA, Panama City FL Mosquiron 2010 Field Trials
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Experimental Design Mosquiron 2010 Field Trials
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Experimental Design Eggs were introduced to treatment tanks approx. every 2 weeks Culex eggrafts were placed directly on water surface at WI, MB, BC and FL sites Once hatching occurred all trts. received a larval liquid food slurry Food slurry was a standard formula consisting of liver powder/brewer’s yeast Food administered 3-4 times/wk. until water was cloudy in appearance Mosquiron 2010 Field Trials
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Marshall - WI siteMeansville - GA site
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Mosquiron 2010 Field Trials Panama City -FL Site
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Mosquiron 2010 Field Trials Portage la Prairie - MB Site
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Mosquiron 2010 Field Trials Abbotsford - BC Site
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Data Collection Treatments were inspected for the presence of live larvae and/or pupae on an as needed basis. Once 3rd and/or 4th instars were present, dips were taken to harvest live larvae. Florida A&M’s PHEREC Standard Mosquito Sampling Method was used at all trial sites. Using a standard mosquito dipper, eight dips were taken per treatment. The number of larvae and/or pupae per dip were counted and recorded. Larvae were returned to the treatment tanks. Once pupae were found, they were transferred to emergence jars for monitoring. Adults that emerged successfully from pupal cases were counted and recorded as successful hatches. Dead adult mosquitoes on the water surface recorded as unsuccessful emergence. Mosquiron 2010 Field Trials
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Observations Using local sources for harvesting Culex eggrafts delayed start of trial in Northerly sites to early July Was exacerbated by a cool wet spring in Canada and WI FL location received very high precipitation (18” rain in July) causing significant dilution of novaluron in cement troughs Conclusions Study results demonstrate Mosquiron formulations provided 100% IE for duration of study at all locations 100% IE observed at FL site with 2x CRD
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