1. Nitric Oxide in Health, Disease, and Therapeutics NO Daniel Kim-Shapiro Department of Physics and Translational Science Center

2. NO deficiency contributes to much pathology and it can be used to treat many diseases NO improves cardiovascular function – Vasodilator – Inhibits platelet aggregation – Anti-inflammatory (reduce ROS) – Rescue endothelial dysfunction (aging) Treatment for pulmonary hypertension Protective against ischemic reperfusion injury Deficient in hemolytic conditions (incl stored blood) 5/19/2015Wake Forest University2

3. 5/19/2015Wake Forest University3 Discovery that Nitrite Vasodilates Cosby et al, Nature Med., 2003

4. Nitrite Therapeutics Sickle Cell Disease Chronic vascular disease

5. Nitrite Therapy via Nitrate Intake

6. 5/19/2015Wake Forest University6 Nitrate Diet and Physical/Cognitive Functional Health High Low This is your brain This is your brain on nitrite

7. “Have you heard about beet juice? The blood-red elixir of the beet is apparently the hottest thing for Olympic athletes looking for a non-illegal performance boost. Soon the world will be caught up in beet juice fever. Too bad it tastes like "sweet dirt.“”"sweet dirt.“

8. Current Projects 5/19/2015Wake Forest University8 Further Studies in Hemoglobin Nitrite reactivity Hemolysis: NO, platelets and ROS Develop compounds to treat Hemolysis Study nitrite in older adults and disease Improve blood transfusion and develop blood substitutes

9. Tools Quantum Gravitational Fragility Tester 3000

10. 5/19/2015Wake Forest University10 Kim-Shapiro Lab and Associates

11. 5/19/2015Wake Forest University11 Funding