1. Sun and Shade Acclimation of the Photosynthetic Apparatus in Gymnostomum aeruginosum Adam Yock 2003-04

2. http://www.scibridge.sdsu.edu/coursemats/introsci/diversity/chlorobionta/chlorobionta_cladogram.jpg

3. Chloroplast

4. Granum (stack of thylakoids) Thylakoid

5. Chloroplast Granum (stack of thylakoids) LHC RC electron transport pathway Sun Photons Thylakoid NADPHNADP + ADP + P ATP PSIPSII

6. Light-Harvesting Complexes (LHC) Photosystematic antennas Contain chl a and b Reaction Centers (RC) Beginning of electron transport chain Contain chl a Introduction

7. Reactions of Light-acclimated Specimens LHC chl a/b

8. Post and Vesk analyzed light adaptability in moss using chl a/b Previous Studies

9. Analyze light adaptability in moss (Gymnostomum aeruginosum) using both a chlorophyll ratio (chl a/b) and a protein ratio (LHCp/RCp). Purpose

10. Peas: Both chl a/b and LHCp/RCp would significantly change Moss: Neither chl a/b nor LHCp/RCp would significantly change Hypotheses

11. 1: p = 6. 2464 x 10 -5 p = 0.8868

12. Table 1: Average Chlorophyll/gram of Fresh Weight

13. Tris-Tricine 10-16% gradient Electrophoresis gel

14. 2:

15. 3:

16. Table 2: Thylakoid Protein Ratios (LHCp/RCp) in Peas

17. Table 3: Thylakoid Protein Ratios (LHCp/RCp) in Moss

18. Explore the apparent discrepancy between the change in chlorophyll ratios and the change in the protein ratios Use at least three thylakoid isolations per specimen Further Study

19. Dr. Verhoeven Ms. Fruen Breck Team Research 2003-04 Acknowledgements

20. Sun and Shade Acclimation of the Photosynthetic Apparatus in Gymnostomum aeruginosum Adam Yock 2003-04

21. Chl a = 12.25 x A 663.6 – 2.55 x A 646.6 Chl b = 20.31 x A 646.6 – 4.91 x A 663.6 Chl a + Chl b = 17.76 x A 646.6 + 7.34 x A 663.6 Absorbance readings entered into the equations: Analysis

22. Chlorophyll Ratios: Pea chl a/b changed significantly (p = 6. 2464 x 10 -5) Agrees with hypothesis Moss chl a/b did not change significantly ( p = 0.8868 ) Agrees with hypothesis Discussion

23. Protein Ratios: Pea LHCp/RCp changed significantly (p = 1.44 x 10 -5 ) Agrees with hypothesis Moss LHCp/RCp changed with marginal significance (p = 7.00 x 10 -2 ) Does not agree with hypothesis Discussion

24. Methods: Collecting Moss/pea Samples Peas: Grown from seed Moss: Harvested from the banks of a small rivulet in St. Paul, MN

25. Prepared -Moss ground in liquid nitrogen -Peas blended in a blended Vortexed and spun in centrifuge (moss twice, peas three times) Aliquoted Isolating Moss/pea Thylakoids

26. Mixed with acetone -Moss ground in acetone -Peas diluted with acetone and vortexed Spun in centrifuge (moss twice, peas once) Read supernatant in spectrophotometer at 663.6 nm and 646.6 nm Measuring Moss Chlorophyll Content

27. Spun in centrifuge Boiled Moss/pea Sample Electrophoresis Preparation

28. Results