1. Announcements Raise your hand if using Pubcrawler?

3. Announcements No recitations tomorrow. Write researcher for mutant DNA yet? If one primer anneals at 50 o C and the other at 60 o C what’s a good temp for your PCR annealing? Raise your hand if using Pubcrawler?

4. If primer #1 is calculated to anneal at 50 o C and primer #2 at 60 o C what’s a good temp to try for your annealing temperature in the first PCR experiment you do? a. 45 o C b. 50 o C c. 55 o C d. 60 o C e. 65 o C

5. Facilitated Diffusion

7. Osmosis (diffusion of water)

8. Water follows solute (salt) HEY!

9. If the membrane is permeable to water and monosaccharides… 1. Which solutes will diffuse into the cell? 2. In which direction will water move? 3. Is the cell’s solution iso, hypo, or hypertonic to the beaker’s? 4. Will the cell die? An artificial cell with an aqueous solution enclosed in a selectively permeable membrane has just been immersed in this beaker.

10. If the membrane is permeable to water and monosaccharides… 1. In which direction will water move? A.Into the cell causing it to swell up B.Out of the cell causing it to shrink C.Equally in both directions An artificial cell with an aqueous solution enclosed in a selectively permeable membrane has just been immersed in this beaker.

11. If the membrane is permeable to water and monosaccharides… 2. Is the cell’s solution iso, hypo, or hypertonic to the beaker’s? A.The cell’s solution is isotonic to the beaker’s B.The cell’s solution is hypotonic to the beaker’s C.The cell’s solution is hypertonic to the beakers An artificial cell with an aqueous solution enclosed in a selectively permeable membrane has just been immersed in this beaker.

12. ATP ADP + P Active Transport binding hydrolysis release

14. Based on the model of sucrose uptake shown, which of the following treatments would increase the rate of sucrose transport into the cell. a. Decreasing extracellular sucrose concentration. b. Decreasing extracellular pH c. Decreasing cytoplasmic pH d. Adding an inhibitor that blocks the creation of ATP. e. Adding a substance that makes the membrane more permeable to H+ cytoplasmoutside cell

15. Based on the model of sucrose uptake shown, which of the following treatments would increase the rate of sucrose transport into the cell. a. Decreasing extracellular sucrose concentration. b. Decreasing extracellular pH c. Decreasing cytoplasmic pH d. Adding an inhibitor that blocks the creation of ATP. e. Adding a substance that makes the membrane more permeable to H+ cytoplasmoutside cell