1. Membrane potential

2. definition It is the difference in electrical potential between the two sides of the membrane surface under resting conditions. The inside is negative relative to the outside of the membrane (polarized state).

3. Measuring cell membrane potential

4. Resting membrane potential (RMP) myelinated nerve& skeletal muscle is -90 mV. The RMP in Medium-sized neuron is -70 mV. The RMP of the cardiac muscle is -60 Mv.

5. Causes of RMP Selective permeability for:Na+KNa-K ATP pump Diffusion potential

6. 1. Selective permeability Inside of the cell: K +. is the main cation &proteins are the main anions. The cell membrane is : It is more permeable to K +, less permeable to Na +,freely permeable to Cl - & impermeable to proteins.

8. Cont ” selective permeability K + ions: Although K + is more concentrated inside the cell, it tends to diffuse outside the cell according to 1-The concentration gradient 2- the high permeability of the cell membrane. However the K+ outflux is limited by : 1-Attraction between K + & intracellular proteins. 2-Repulsion between K + & Na + outside cell membrane. 3- K + -Na + pump which derive K + inside the cell

9. Na + ions: Although Na+ is more concentrated outside the cell, it tends to diffuse inside the cell according to: 1-The concentration gradient. 2-Electrical gradient. However the Na+ influx is limited by : 1-the low permeability of the cell membrane to Na+. 2.Na+ -K pump which pump Na outside the cell. Cont ” selective permeability

10. 2.Na-K ATP pump Pumps 3 Na+ outside the cell for every 2 K+ Electrogenic pump

11. Action potential A short-term change in the electrical potential on the surface of a cell (e.g. a nerve cell or muscle cell) in response to stimulation, and spread rapidly along the nerve fiber membrane.

13. The Nobel Prize in Physiology or Medicine (1963) “for their discoveries concerning the ionic mechanisms involved in excitation and inhibition in the peripheral and central portions of the nerve cell membrane” Alan Lloyd Hodgkin Andrew Fielding Huxley

14. Basic Electrophysiological Terms oStimulus: a sudden change of the (internal or external) environmental condition of the cell. oincludes physical and chemical stimulus. oThreshold (intensity): the lowest or minimal intensity of stimulus to elicit an action potential

15. Basic Electrophysiological Terms :  Polarization: a state in which membrane is polarized at rest, negative inside and positive outside.  Depolarization: the membrane potential becomes less negative than the resting potential (close to zero).  Hyperpolarization: the membrane potential is more negative than the resting level.  Repolarization : restoration of normal polarization state of membrane.

17. Phases of action potential 1.resting stage 2 depolarization stage 3.repolarization stage

18. 1.resting stage The membrane is polarized. all Na+ and K+ gated channels are closed.

19. 2.depolarization stage Depolarization phase the voltage gated fast Na+ channels open partially & when it reaches - 55mV :Na+ channels are fully open and the Na+ moves inside the cell.

20. Activation gate Inactivation gate

21. "for their discoveries concerning the function of single ion channels in cells" The Nobel Prize in Physiology or Medicine (1991) Erwin NeherBert Sakmann

22. 3.Repolarization stage Repolarization hyperpolarizati on

23. Properties of the Action Potential “All or none” phenomenon A threshold or suprathreshold stimulus applied to a single nerve fiber always initiate the same action potential with constant amplitude, time course and propagation velocity. Propagation Transmitted in both direction in a nerve fiber

24. Inhibition of action potential Local anesthetics: Proacaine and Tetracaine. Work on gate of Na channels: difficult to open.

25. Refractory periods 1.relative 2.Absolute.

27. Slide 3 of 28

28. Any questions?