1. Science, Technology, Engineering, and Math all rolled up into iGEM Ung.igem.org/High_School_Division

2. Foundation Science for iGEM Synthetic Biology take molecular genetics and apply engineering principles: Standardization: Development of systems that are standards in the biological world ex. Restriction enzyme systems, promoters/translational unit systems Decoupling: Separating a complex system into simple systems that work together ex. Building a fragrance free chassis, building tolerance to salt water, sense arsenic, and fragrance system. When they are put together they can make a bacteria that can tolerate salt water, sense arsenic and release a fragrance in the presence of arsenic. Abstraction: Systems can be thought of in a hierarchy in two forms Work in one level and disregard the other levels Allow for the exchange between levels ex. Relationship between RBS and ORF Synthetic Biology

3. Teams design, engineer, and build a biological machine from standard parts Team structure: All students should have beginning biology Select students that are willing to work after school May want students that have a computer background Timeline Flexible schedule dependent on the students Afterschool activity Correlate with curriculum in a class Registration is from Mid August to Mid November register at http://ung.igem.org/Main_Page Team abstract are due in May with registration to the jamboree. Declare a track for the jamboree. Teams will be judged based on the track chosen. Environmental, Health and Medicine, Informational Processing, Food and energy, Foundational Advance, Manufacturing, New Application, Software Tools Jamboree is last Saturday in June Budget If you have the equipment, $300-$1,000 to do the lab work iGEM: International Genetically Engineered Machine

4. Project idea: Project brain storming session relevant to a need in society Ethics of synthetic biology: Just because we can does not mean we do Pick a Biological system Research the host: based on the complexity and mentors available to your team Make sure that you have competent cells to work with. Most cells that come from kits are considered competent. You can look on the suppliers website to find out. Research existing biological circuits Team members research current parts that my be available for their system. These parts can be from the host or form the registry. Design the new circuit to manipulate the host Team members design the plasmid’s circuit to allow the host to become the machine. This can be a multiple step system where students set goals to achieve during the building session. Students research plasmid and part design to make their robot. They research current parts and systems to help with the designing. Community outreach program: Team members talk about their project to the community. Hook activities smelly bacteria or photogenic bacteria Footsteps of an iGEMer: Fall Semester

5. Building of the machine: Teams build the machine using parts Insertion into a plasmid Transformation into the host cell Standardization of results Mathematically compute what the results mean to the user ex. Level of florescence to concentration of promoter Celebration of success Jamboree Teams get together to display their results at the poster session. Then teams do a 20 minute presentation to judges. October Teams can be volunteers for the Americas regional in Indianapolis Teams present their posters during the poster session at the Americas Regional Footsteps of an iGEMer: Spring Semester

6. Search for Parts Natural DNA Parts from the Registry

7. iGEM parts that are received after registration Extraction of the parts form the genome Using primers ordered from a vendor Write a lab that has the part and request that part In the research of the part, students will read about labs performing testing. Students can contact the lab and ask for a sample. Acquire the Parts

8. Restriction Enzymes 3 A Assembly Method

9. Primers and homologies C:\Users\William Schini\Videos\The Gibson Assembly Song.mp4 Gibson Assembly

10. Community outreach: 2012 each team will be asked to develop and implement a community outreach program. Students can talk with local groups about their project. Develop activities and perform them with middle school students. Ethics of Synthetic Biology (GMO): Team leaders can discuss the ethics of GMO’s and understand the uses of GMO’s Create new parts for submission into the registry Natural Engineered Create new plasmid backbone Submit work for publishing Other avenues Benefits for the student: Any team competing in the jamboree will be able to present their poster at the collegiate regional. Potentially to the final jamboree. Students get to meet with leading scientists in the field of molecular genetics, synthetic biology, biology, math, and engineering. Connections developed through contact with mentors and scientists. Colleges do notice iGEM on applications, especially schools of medicine.