1. The Effectiveness of Disinfectants on Pseudomonas fluorescens
Michelle Alvarez, Kadija Bangurah &
Paige Stox
Department of Microbiology
North Carolina State University

2. Inspiration
How effective are your everyday household cleaners against bacteria?
Does it really kill 99% of germs?

3. Pseudomonas fluorescens
Gram-Negative
Rod- Shaped
Multiple flagella
Soil and marine environments
Obligate aerobe
The optimum growth temperature is degrees Celsius
Produces the antibiotic Mupirocin which treat skin, ear, and eye disorders
Nonpathogenic and lack virulence factors

4. Problem/ Hypothesis
Problem: Which disinfectant will be the most effective on killing Pseudomonas fluorescens?
Hypothesis: Clorox Bleach Gel will be the most effective on killing Pseudomonas fluorescens.

5. (9) Disinfectants used:
Method/Materials
(9) Disinfectants used:
Vinegar- Acetic Acid
Hand Sanitizer- ethyl alcohol
Clorox Gel Bleach- Sodium Hypoclorite
Pine Sol- Pine Oil
Hand Soap- Sodium Laureth Sulfate
Clorox Wipes- n- Alkyl-Dimethyl Benzyl Ammonium Chlorde
Lysol Spray- Hydrogen Peroxde
Dish Detergent- Triclosan
Shampoo- Ammonium Laureth Sulfate
Bioscreen C instrument
10x Nutrient Broth
dH20
Culture: Pseudomonas fluorescens
Series of Dilutions:
1: 20
1: 30
1: 50
1: 100
1: 1,000
1: 10,000
1: 1,000,000
Optimal Density is measured by the bioscreen C instrument for 60 hours

6. Preliminary Trial 1

7. 1:10,000 dilution

8. 1:100,000 dilution

9. Conclusion
The top two disinfectants that worked the best at inhibiting bacteria growth were Pine Sol and Clorox Bleach Gel.
At 1:100,000 dilution our culture started to grow and both disinfectants were no longer capable of killing our bacteria. Therefore our hypothesis was supported!

10. Why?
Pine Sol:
Pine Oil
Clorox Bleach Gel:
Sodium Hypochlorite

11. Questions?