1. Kingdom Monera - Bacteria Kingdom Monera - Bacteria Reproduction and Ecological Roles www.onacd.ca

2. Bacterial Reproduction Asexual reproduction: BINARY FISSION BUDDING

3. Binary Fission Most bacterial cells will grow to a fixed size and will then reproduce by binary fission Note: equal division of cytoplasm and genetic material 1. A single bacterial cell begins to make copies of its genetic material 2. The cell membrane begins to elongate and pulls the genetic material in two different directions 3. The cell membrane grows inward and separates the cells. The cells are genetically identical to one another.

4. Bacterial cells undergoing different stages of binary fission

5. Budding Results in uneven division of cytoplasm and even division of nuclear material More common with fungi, not bacteria

6. Benefits and disadvantages of sexual and asexual reproduction ProsCons AsexualOnly 1 organism is necessary -very efficient (20 min life cycle) Get exact copy (clone) therefore, no species diversity and 1 thing will kill all the bacteria SexualExchange of DNA, therefore high diversity If there is only 1 individual, it will die with no offspring

7. Conjugation – Sexual Reproduction A primitive form of sexual reproduction where there is exchange of chromosome (red) material (genetic material) between two bacterial cells through a structure called a pili. Enlarged Examples of bacteria that are known to undergo conjugation include: E. coli, Salmonella

8. Studying bacteria Labs use 2 different growth mediums. (substances that bacteria grow in)

9. Petri dish containing agar Bacteria are “streaked” across agar – gelatinous substance made from algae

10. Innoculating loop used to transfer bacteria to the agar plate

11. “ Slant Test-tube” containing agar with bacteria growing on the surface

12. Nutrient broth Test-tubes filled with: Blood Beef juice Other stuff

13. Ecological Roles of Bacteria nitrogen fixation in the soil next the roots of some plants. -plants obtain an easily absorbable form of nitrogen needed for growth. degrade some organic compounds -aid in cleaning up oil spills

14. Beneficial Roles of Bacteria symbionts in humans and other organisms. over 1000 types of bacteria in the average human stomach and intestines that contribute to: -immunity, vitamin absorption and synthesis, nutrient conversion and fermentation. (probiotic supplements contain many of these bacteria..)

15. Beneficial Roles of Bacteria Used in preparation of fermented foods ex. Yogurt, cheese, soy sauce… Used in the study of genetics, molecular biology and biochemistry because of their ability to reproduce so quickly

16. Some beneficial bacteria Escherichia coli – produces vitamin K in large intestine – necessary for blood clotting Lactobacillus – yogourt Streptococcus – to sour milk Clostridium acetobutylicum – to make acetone and butanol at an industrial scale

17. Antibiotics made by bacteria Streptomyces – make antibiotics including: Amphotericin Kanamycin Neomycin Streptomycin Tetracycline

18. Harmful bacteria - Pathogenic Tuberculosis Meningitis Botulism Cholera Syphilis Gonorrhea Diphtheria Black plague Gangrene

19. Treatments for Bacterial Infections 1. Antitoxin – to neutralize a chemical made by bacteria that produce toxins - ie. Botulism 2. Antibiotics – chemicals produced by other organisms that kill the bacteria in a form of chemical warfare - ie. Penicillin (a fungus) makes antibiotics

20. 3. disinfectant – to kill all bacteria on surfaces 4. antiseptic – to kill most bacteria on the body surface

21. Other ways to slow down growth or kill bacteria in food 1. Keep cold/freeze – slow down metabolism 2. Can – keep air out – slows growth – heat kills enzymes 3. Dry – take away water – needed for metabolism 4. Cure – the salt draws away moisture from the food – the smoking dries the food

22. 5. Preservatives – chemicals that kill bacteria 6. Salt – draw away moisture 7. Sugar – draw away moisture

23. Effective use of antibiotics Take only when necessary – don’t work on viruses Take for total length of time – endospores (protective coat) forms around bacteria for protection and when the conditions are good again, the bacteria becomes active again Take complete dose or else you increase risk of antibiotic resistant bacteria

24. Antibacterial Agents and Resistance www.onacd.ca

25. Antibiotics Definition: a substance produced by a microorganism that restricts the growth of another microorganism Where do antibiotics come from? many are produced from other living organisms example. Penicillin is collected from the fungi penicillium (above) Streptomycin comes from the bacteria streptomyces some antibiotics are modified chemically and some are produced synthetically Penicillium fungi : produces the antibiotic penicillin

26. How do antibiotics work? Antibiotics that target a wide range of bacteria are termed broad spectrum. Those that target a narrow range are termed narrow spectrum. Some antibiotics actually destroy bacteria (inhibit cell processes) whereas others keep the bacterial cell from reproducing (inhibit binary fission) Above: Wafers containing antibiotics are placed on an agar plate of bacteria. The cloudy areas show bacterial growth. Circles of poor bacterial growth show that bacteria will not grow in the presence of that antibiotic.

27. Antibiotic Resistance As close as possible to 100% of harmful bacteria must be killed off when taking a round of antibiotics. If not, the surviving bacteria will most likely carry genes that are resistant to the initial antibiotic and will then begin to multiply. Failure to take entire prescribed dosages of antibiotics can lead to the growth of antibiotic resistant bacteria. When antibiotics are administered when they are not needed we are exposing more bacteria to selection for resistance. Improper usage and dosaging of antibiotics also contributes to the appearance of resistant bacteria. NOTE: Remember as well that the genes for antibiotic resistance can also be transferred to other normal bacteria through the conjugation.

28. Staphylococcus aureus bacteria are one of the types of bacteria normally found existing on human skin surface. They are capable of causing skin infections in those with weakened immune systems. (see picture to left) Skin infections caused by these bacteria were treated successfully in the 1940’s and 50’s with penicillin. Presently, almost all strains are resistant to penicillin which leaves only a small section of drugs (vancomycin) to treat it. The picture to the left shows a new form of S.aureous that is beginning to show resistance to vancomycin.

29. Antiseptics Definition: Antimicrobial substances that are applied to living tissue (skin) to inhibit the growth of bacteria Some destroy bacteria (germicides) and some inhibit the growth of bacteria Overuse can lead to increased dangerous resistant strains of bacteria

30. Common Types of Antiseptics Alcohol (surgical alcohol) –Used to disinfect skin before injections are given Boric Acid –Used in yeast infection treatments, cold sore medications, burn cream and eye contact solution (although not very effective) Chlorhexidine Gluconate –Used in gingivitis treatment Hydrogen peroxide –Used for cleaning of wounds (although antibacterial soap and water is actually more effective) Iodine –Used in pre and post operative cleaning and has a very wide scope of antimicrobial activity Phenol compounds –Used in pre operative hand washing, mouth washes and throat lozenges Natural antiseptics –Includes saliva, tears, breast milk and gastric acids

31. Disinfectants Definition: antimicrobial agents applied to non-living objects that destroy microorganisms (including bacteria) Many disinfectants are harmful (toxic) to humans, animals and the environment Overuse can lead to resistant strains of bacteria

32. Common Types of Disinfectants Alcohol Wide spectrum, highly effective and non-corrosive but are a fire hazardWide spectrum, highly effective and non-corrosive but are a fire hazard Oxidizing Agents (ex. Chlorine, peroxide, iodine…) Destroys the cell membrane of bacteriaDestroys the cell membrane of bacteria Commonly used inCommonly used in Bleach, swimming pool cleaners, drinking water treatment…)Bleach, swimming pool cleaners, drinking water treatment…) UV light Can be used in dental tool disinfection or for cleaning any other non-porous surfacesCan be used in dental tool disinfection or for cleaning any other non-porous surfaces